辣椒素对人大细胞肺癌细胞NCI-H460增殖和迁移与侵袭能力的影响

摘要

Objective To investigate effects of capsaicin on proliferation,migration and invasion of human large cell carcinoma NCI-H460 and discuss the possible mechanisms by which capsaicin inhibits non-small cell lung cancer. Methods NCI-H460 cells were cultured in vitro and treated with capsaicin at various concentrations. Colony formation assay,wound healing assay,and transwell chamber invasion assay were used to detect the effects of capsaicin on proliferation,migration and invasion of NCI-H460 cells. Western blotting was used to detect the protein expression of E-cadherin,N-cadherin,Vimentin and Snail. Results The rate of colony formation of 100,200,300 μmol.L-1 of capsaicin were (91.17±24.38)%,(43.22±12.91)% and (15.71±4.26)%,respectively,the control group was (99.53±21.25)%.the rate of migration of NCI-H460 cells of 25,50, 100 μmol.L-1 of capsaicin were (85.83±17.43)%,(37.92±10.16)% and(21.08±6.39)%,respectively,the control group was (93.04±20.23)%.The number of invasion of NCI-H460 cells of 25,50,100 μmol.L-1 of capsaicin were (99±18.2),(75± 17.9) and( 52 ± 14. 1 ) , respectively, the control group was ( 107 ± 20. 4 ) . The middle and high-dose capsaicincould inhibit proliferation,migration and invasion of NCI-H460 cells ( P<0.05) ,and E-cadherin expression level was significantly up-regulated and N-cadherin, Vimentin, Snail expression level was significantly down-regulated ( P<0. 05 ) . Conclusion Capsaicin can inhibit proliferation,migration and invasion of NCI-H460 cells,the mechanism may be related to up-regulation of E-cadherin and down-regulation of N-cadherin,Vimentin and Snail expression levels.%目的：观察辣椒素对人大细胞肺癌细胞NCI-H460增殖、迁移与侵袭能力的影响，探讨其抗非小细胞肺癌的可能机制。方法体外培养NCI-H460细胞，以不同终浓度辣椒素进行处理。采用平板克隆形成实验、划痕修复实验、Transwell小室细胞侵袭实验，观察辣椒素对NCI-H460细胞增殖、迁移与侵袭的影响，并采用Western blot分析检测E-cadherin和N-cadherin、Vimentin、Snail 的表达。结果100，200，300μmol•L－1辣椒素克隆形成率分别为(91.17±24.38)％，(43.22±12.91)％和(15.71±4.26)％，对照组为(99.53±21.25)％；25，50，100μmol•L－1辣椒素对NCI-H460细胞迁移率分别为(85.83±17.43)％，(37.92±10.16)％和(21.08±6.39)％，对照组为(93.04±20.23)％；NCI-H460细胞侵袭穿膜细胞数分别为(99.0±18.2)，(75.0±17.9)和(52.0±14.1)，对照组为(107.0±20.4)；中、高浓度辣椒素可显著抑制NCI-H460细胞增殖、迁移与侵袭能力(P<0.05)，显著上调E-cadherin表达水平，并显著下调N-cadherin、Vimentin、Snail表达水平( P<0.05)。结论辣椒素能明显抑制NCI-H460细胞增殖，降低迁移和侵袭能力，其机制可能与上调E-cadherin表达并与其下调N-cadherin、Vimentin、Snail表达有关。