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PREPARATION FOR INHIBITING PROLIFERATION OF LUNG CANCER CELL WITH RNAi SEQUENCE WITH RESPECT TO MGC5627 GENE, METHOD OF MANUFACTURING THE SAME AND METHOD OF INHIBITING PROLIFERATION OF LUNG CANCER CELL THEREBY
PREPARATION FOR INHIBITING PROLIFERATION OF LUNG CANCER CELL WITH RNAi SEQUENCE WITH RESPECT TO MGC5627 GENE, METHOD OF MANUFACTURING THE SAME AND METHOD OF INHIBITING PROLIFERATION OF LUNG CANCER CELL THEREBY
PURPOSE: Provided is a preparation for inhibiting the proliferation of lung cancer cells which expresses RNAi with respect to MGC5627 genes, and effectively inhibits the growth of the lung cancer tissues, and the colony formation and the anchorage independency thereof. CONSTITUTION: The lung cancer cell proliferation inhibitor comprises the RNAi sequence identified by SEQ ID NO 2 for inhibiting the gene expression of MGC5627 identified by SEQ ID NO 1 to treat lung cancers. The RNAi sequence is expressed by the human U6 promoter cassette. The RNAi expression vector with the concentration of 2 micro grams/1x100000 is introduced into the lung cancer cell together with cationic liposome of 1-20 micro grams/1x100000 several times for 4-15 days.
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机译:目的:提供一种抑制肺癌细胞增殖的制剂,其相对于MGC5627基因表达RNAi,并有效抑制肺癌组织的生长及其集落形成和锚定独立性。组成:肺癌细胞增殖抑制剂包含由SEQ ID NO 2鉴定的RNAi序列,用于抑制由SEQ ID NO 1鉴定的MGC5627的基因表达,以治疗肺癌。 RNAi序列由人U6启动子盒表达。将浓度为2微克/ 1x100000的RNAi表达载体与1-20微克/ 1x100000的阳离子脂质体一起多次引入肺癌细胞,持续4-15天。
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