β3-肾上腺素能受体对心室重塑大鼠钙调磷酸神经酶信号通路的调节及益气温阳活血方的干预作用

摘要

Objective To study effects of Yiqiwenyanghuoxue decoction onβ3‐AR regulating CaN sinaling path‐way in the myocardial remodeling of rat with chronic heart failure .Method The model rats with chronic heart failure were injected with isoprenaline (ISO) .Cardiac structure and function was tested by echocardiography ,NFAT3 pro‐tein activity was measured by ELISA ,mRNA and protein atβ3‐AR ,CaN and NFAT3 were determined and analyzed by RT‐PCR and western blot (WB) test respectively .Result Compared with the normal group ,those were signifi‐cantly higher at LVPW ,IVS ,NFAT3 protein activity ,as well as mRNA and protein inβ3‐AR ,CaN and NFAT3 were significantly higher in ISO‐injected group ,but EF % was significantly lower (all P<0 .05) ,but these change can be attenuated by decoction of yiqiwenyanghuoxue ,compared with the normal group and ISO‐injected with Carve‐diol group ,there were no statistical significantly differences in ISO‐ injected with Yiqiwnyanghoxue group(all P>0 .05) .Conclusion Yiqiwenyanghuoxue decoction may reverse the myocardial remodeling through inhibiting β3‐AR and CaN sinaling pathw ay .%目的：探讨β3‐肾上腺素能受体（β3‐AR）对心室重塑大鼠钙调磷酸神经酶（CaN ）信号通路的调节及益气温阳活血方的干预作用。方法通过异丙肾上腺素（ISO ）诱导慢性心力衰竭动物模型。心脏超声检测心脏结构及功能，酶联免疫吸附法检测活化的T细胞核因子3（NFAT3）核蛋白活性，逆转录聚合酶链反应（RT‐PCR）法以及蛋白免疫印记法（Western‐blot法）检测β3‐AR、CaN、NFAT3 mRNA及蛋白质表达水平。结果模型大鼠左室后壁厚度（LVPW）、室间隔厚度（IVS）明显升高，射血分数（EF％值）则明显降低，与正常组比较差异有统计学意义（均P＜0．05）；NFAT3核蛋白活性，β3‐AR、CaN、NFAT3 mRNA和蛋白质的表达水平明显升高，与正常组比较差异有统计学意义（均 P＜0．05）。益气温阳活血方可抑制慢性心力衰竭大鼠 NFAT3核蛋白活性，β3‐AR、CaN、NFAT3 mRNA和蛋白质的表达水平，提高 EF％值、降低 IVS及 LVPW 厚度，与模型组比较差异有统计学意义（均 P＜0．05），而与正常组及卡维地洛治疗组比较差异无统计学意义（均 P＞0．05）。结论益气温阳活血方可通过阻断慢性心力衰竭大鼠过分激活的β3‐AR ，抑制钙调磷酸神经酶信号通路逆转心室重塑。