间接酶联免疫吸附测定河豚鱼中河豚毒素∗

摘要

An indirect ELISA method was developed to monitor tetrodotoxin in puffer and assess the security risk level of the puffer. The indirect ELISA method ensured the safety of puffer fish consumption and monitoring of tetrodotoxin in puffer fish. The indirect ELISA method was sensitive, the quantification limit of the method was 2 μg/kg with satisfactory sensitive. The recovery of tetrodotoxin was 82. 4% ~92. 5% in the range of 2~135 μg/L. The method was rapid and simple, met the needs of practical work.%为有效确保食用河豚鱼的安全性，进一步监测河豚鱼中河豚毒素，本方法应用间接酶联免疫吸附测定河豚鱼中河豚毒素的含量，评估河豚鱼的安全风险程度。实验结果表明，间接酶联免疫吸附法测定河豚毒素灵敏度高，方法的在线性范围为2~135μg/L内有良好的线性关系，且方法的回收率达到82．4%~92．5%，检出限能达到2μg/kg。本方法快速、简单，完全满足实际工作的需要。