腹腔注射氢气与腹腔氢水对大鼠氢气代谢的影响

摘要

目的 比较腹腔注射氢气与氢水对鼠氢气代谢的影响.方法 20只SD大鼠,在监测呼气氢气基础值后,均先后腹腔注射氢气及饱和氢气生理盐水1 ml/100 g及2 ml/100 g.记录不同给氢方式呼气氢气浓度的达峰时间、峰浓度,计算半衰期和曲线下面积,比较不同给氢方式对呼气氢气浓度的影响.结果 腹腔注射1 ml/100 g氢水、氢气、达峰时间(Tmax)分别为(3.48±1.54)min、(15.85±7.24)min,注射2 ml/100 g氢水、氢气分别为(5.48±1.88)min、(21.25±11.65)min;1 ml/100 g氢水、氢气峰浓度(Cmax)分别为(35.04±12.43)ppm、(44.43±16.13)ppm,2 ml/100 g氢水、氢气分别为(49.45±18.89)ppm、(62.82±23.74)ppm,同剂量不同方式比较及同方式不同剂量比较,差异有统计学意义(P＜0.01);单倍剂量氢水、氢气半衰期(T1/2)分别为(8.37±3.22)min、(125.08±73.68)min,双倍剂量氢水、氢气分别为(11.21±4.84)min、(168.46±85.77)min;1 ml/100 g氢水、氢气较基础值增加的曲线下面积(AUC)分别为(279.43±161.67)ppm/min、(4 249.87±1 689.74) ppm/min,2 ml/100 g氢水、氢气分别为(384.03±172.25)ppm/min、(6 834.04±2471.67)ppm/min.可见同剂量不同方式比较及同方式不同剂量比较差异有统计学意义(P＜0.01).结论 腹腔注射氢水、氢气均可迅速提高呼气氢气浓度,存在量效关系.腹腔注射氢气能长时间维持呼气氢气高浓度.%Objective To compare; thc; e;ffe;cts of intrapcritoncat injection vvith saturated hydrogen satine; or hydrogen gas on hydrogen metabotism in rats. Methods Tvvcnty SD rats were; te;ste;d the; basis concentration of e;xhate;d hydrogen. Then att of them were; successivety supptied intraperitoneat saturated hydrogen satine; or hydrogen gas of 1 mt/KX) g and 2 mt/KX) g. To record the; peak time; of e;xhate;d hydrogen concentration( Tmax ) of different hydrogen-supptied ways, the; max e;xhate;d hydrogen concentration( Cmax ),and to catcutate hatf-tife;( T1/2 ) and area under the; curve( AUC ). A compaiison of the; e;ffe;cts of different hydrogen-supptied ways on e;xhate;d hydrogen concentration was done;. Resutts With intraperitoneat injection of 1 mt/KX) g of hydrogen satine; vs hydrogen gas,Tmax were ( 3.48 ± 1. 54 )min vs ( 15. 85 ±7. 24 )min. Tmax were ( 5.48 ± 1. 88 )min vs ( 21.25 ± 11.65 )min with intraperitoneat injection of 2 mt/KX) g of hydrogen satine; vs hydrogen gas. Cmax were ( 35. 04 ± 12. 43 )ppm vs ( 44.43 ± 16. 13 )ppm in intraperitoneat saturated hydrogen satine; vs hydrogen gas of 1 ml/100 g and ( 49.45 ± 18. 89 )ppm vs ( 62. 82 ±23.74 )ppm of 2 mt/KX) g; Thorn was significant difference; among diffe;re;nt hydroge;n-supptied ways of the; same; dose; as wett as among etiffe;re;nt dose;s with the; same; hydroge;n-supptied way( P ＜0.01 ) T1/2 we;re; ( 8.37 ±3.22)min vs ( 125.08 ±73.68 )min in intrapritoncal satiuate;d hydroge;n satine; vs hydrogen gas of 1 ml/100 g and ( 11. 21 ±4. 84 )min vs ( 168.46 ± 85. 7 )min of 2 ml/100 g. The; increased AUC were; ( 279. 43 ± 161. 67 )ppm/min vs ( 4 249. 87 ±1 689. 74 )ppm/min in intraperitoneat saturated hydrogen satine; vs hydrogen gas of 1 ml/100 g and (384.03 ±172. 25)ppm/min vs (6 834. 04 ±2 471.67 )ppm/min of 2 mt/100 g;There; was significant difference;among different, hydrogen-supptied ways of the same dose as wett as among different, doses in the same hydrogen-supptied way( P ＜ 0. 01 ). Conctusion Intraperitoneat injeetion with hydrogen satine or hydrogen gas can rapidty increase exhated hydrogen eoneentration in rats with dose-effeet retationship. Intraperitoneat injeetion with hydrogen gas can maintain a high-dose exhated hydrogen eoneentration for a tong time.