补肾调肝清心方对围绝经期抑郁症睡眠障碍大鼠模型海马5-羟色胺1A受体、5-羟色胺2A受体的影响

摘要

Objective Observe the effect of Bushen Tiaogan Qingxin decoction on hippocampal 5-HT1A and 5-HT2A receptor of Perimenopausal depression model rats. Methods 60 perimenopausal rats were randomly divided into 5 groups, 12 rats in each group. The normal group ( A); perimenopausal de-pression sleep disorder model group ( B ); perimenopausal depression sleep disorder progynova treatment group (C); perimenopausal depression sleep disorder mirtazapine treatment group (D); perimenopausal depression sleep disorder TCM treatment group ( E) . A group is perimenopausal normal rats, the other 4 groups were under 18 days of unpredictable chronic stress. After the last administration, the rats were kill-ed by cervical dislocation to get the hippocampus and test the 5-HT1A receptor mRNA and 5-HT2A recep-tor mRNA by fluorescence quantitative detection, test 5-HT1A and 5-HT2A receptor expression by RT-PCR and Western blot. Results Use Western blot to test the hippocampus and immunofluorescent to test the CA1 region of the hippocampus showed: 5-HT1A receptor expression in hippocampus had no significant differences (P>0. 05). The expression level of 5-HT2A receptor of model group was significantly lower than that of normal group (P<0. 05), the treatment group improved the expression level of 5-HT2A recep-tor, and there were significant differences (P<0. 05). RT-PCR showed:5-HT1A receptor mRNA level in hippocampus showed no significant differences in each group (P>0. 05), and 5-HT2A receptor mRNA level of the model group was significantly lower than that of normal group ( P<0. 01 ) , mirtazapine group compared to the model group had significant differences (P<0. 001), TCM group compared with the model group had significant differences (P<0. 05). Conclusion Bushen Tiaogan Qingxin decoction can increase the expression of mRNA and protein levels of 5HT2A receptor, and immunofluorescence results showed that the effect happened in the CA1 region of hippocampus, and is close to progynova and mirtazapine.%目的：观察补肾调肝清心方对围绝经期抑郁症睡眠障碍模型大鼠海马5-羟色胺1A受体(5-hydroxytryptamine 1A receptor,5-HT1AR)、5-羟色胺2A 受体(5-hydroxytryptamine 2A recep-tor,5-HT2AR)的影响。方法将60只围绝经期大鼠按体重随机分为5组,每组12只：围绝经期正常对照组( A)；围绝经期抑郁症睡眠障碍模型组( B)；围绝经期抑郁症睡眠障碍补佳乐治疗组( C)；围绝经期抑郁症睡眠障碍米氮平治疗组( D)；围绝经期抑郁症睡眠障碍中药治疗组( E)；A组为围绝经期正常大鼠,其余4组进行18天不可预知慢性应激。于应激后对造模动物进行72小时连续快速眼相睡眠剥夺( REM)。每天在应激前1小时按组给药。在末次灌胃24小时后,颈椎脱臼法处死大鼠,取大鼠海马,分别用荧光定量检测海马5-HT1ARmRNA和5-HT2ARmRNA、蛋白印迹和免疫荧光检测海马5-HT1AR和5-HT2AR的表达。结果蛋白印迹检测( western blot)海马及免疫荧光检测海马CA1区均显示：海马区5-HT1AR表达水平各组无明显差异(P>0．05),而5-HT2AR表达水平上,模型组显著低于正常组(P<0．05),治疗组提高了5-HT2AR的表达水平,且与模型组相比存在显著性差异(P<0．05)；RT-PCR显示：海马区5-HT1AR mRNA水平各组无明显差异(P>0．05),而5-HT2AR mRNA水平,模型组明显低于正常组(P<0．01),米氮平组较模型组有极显著性差异(P<0．001),中药组较模型组有显著性差异(P<0．05)。结论补肾调肝清心方能提高5-HT2AR的mRNA及蛋白水平的表达,免疫荧光的结果表明在海马CA1区发挥作用,表明补肾调肝清心方可以通过调节海马CA1区5-HT2AR表达来改善睡眠障碍。