响应面法优化发芽大豆富集多肽的培养液组分

摘要

以浸润方式培养大豆,考察赤霉素（GA3）、CaCl2等10种添加物对发芽大豆多肽富集的影响,对呈显著正效应的添加物进行最适浓度优化,并探讨各组分浓度对发芽大豆多肽富集的影响。结果表明：GA3、CaCl2、AlCl3和MnSO4对发芽大豆多肽富集有显著正效应;Box-Behnken试验结果显示,发芽大豆中多肽含量与培养液组分浓度之间的二次多项模型显著（P〈0.05）,可预测发芽大豆中多肽的富集;其中GA3、CaCl2、AlCl3和MnSO4最适浓度分别是61.00μmol/L、11.20mmol/L、10.13mmol/L和12.17mmol/L,在此条件下培养的发芽大豆中多肽含量达到162.57mg/g,分别是对照和原料大豆的2.10倍和3.97倍,由此表明优化后的培养液组分能显著提高发芽大豆多肽含量。%The effects of 10 components in the culture solution on the accumulation of polypeptides after 5 days of soybean germination by means of infiltration were investigated.Using Plackett-Burman experiment design,GA3,CaCl2,aluminium chloride（AlCl3） and manganous sulfate（MnSO4） were identified as main factors that significantly positively affect polypeptide accumulation.The quadratic polynomial regression model for polypeptide content in germinated soybean versus the four variables established based on Box-Behnken experiment design was statistically significant（P0.05） and could allow the prediction of polypeptide accumulation.The optimal concentrations of GA3,CaCl2,AlCl3 and MnSO4 in the culture solution were 61.00 μmol/L,and 11.20,10.13,12.17 mmol/L,respectively.The polypeptide content in germinated soybean cultured under these conditions was 162.57 mg/g,which was 2.10-fold and 3.97-fold higher than the control and non-germinated soybean,respectively.Therefore,the optimized culture solution can significantly increase polypeptide content in germinated soybean.