大豆抗癌肽的初步提取及抗癌活性

摘要

确定大豆抗癌肽最佳提取条件,并对其抗癌活性进行检测。大豆经乙醚、石油醚或正己烷脱脂预处理后,分别对大豆粉过筛目数、脱脂粉添加量和超声波处理时间进行三因素三水平正交试验设计,通过对胃癌细胞SGC-7901生长抑制率的影响,探讨大豆抗癌肽的最佳提取条件;采用四噻唑蓝比色法（MTT法）对不同处理条件下的大豆提取物进行抗癌活性检测。结果表明：大豆抗癌肽最佳提取条件为乙醚脱脂、过80目筛、脱脂粉添加量3g/15mLPBS缓冲液（pH7.4）、超声波处理时间80min,经验证实验,此条件下癌细胞增殖抑制率达到39.03%。%Dried soybean powder was defatted with either petroleum ether,n-hexane or ethyl ether before the extraction of anticancer peptides with PBS buffer（pH 7.4） under ultrasonic assistance.In order to maximize the inhibitory rate of anticancer peptides against human gastric cancer cell line SGC-7901,a three-factor,three-level orthogonal array design was used to optimize three operating conditions including material particle size,solid-to-liquid ratio and ultrasonic treatment time.Anticancer activity evaluation of anticancer peptides extracted from defatted soybean was carried out using MTT assay.The optimal extraction conditions were determined as follows： material particle size 80 mesh,solid-to-liquid ratio 1：5（g/mL） and ultrasonic treatment time 80 min.The inhibitory rate of the extract under these conditions against the proliferation of human gastric cancer cell line SGC-7901 was 39.03%.