黑酵母β-1,3-1,6-葡聚糖对小鼠肿瘤及免疫功能的影响

摘要

目的：探讨黑酵母β-1,3-1,6-葡聚糖对S180腹水移植瘤荷鼠瘤体生长以及免疫功能的影响。方法：建立BALB/c小鼠S180腹水移植瘤模型，每日给予β-1,3-1,6-葡聚糖干预，并测量瘤体积。21d后，检测小鼠血清中10种细胞因子IL-2、 IL-4、 IL-6、IL-8、 IL-10、IL-12、 IFN-γ、TNF-α、TNF-β和G-CSF的水平，以及CD4、CD8、补体C3、IgG和 IgM的含量。结果：β-1,3-1,6-葡聚糖能显著抑制S180移植瘤的生长(P＜0.01)。与空白对照组比较，多糖灌胃组小鼠血清中细胞因子IL-2、IL-4、IL-6、IL-8、IL-10、IL-12、TNF-α、TNF-β、IFN-γ和G-CSF水平显著上升(P＜0.01，P＜0.05)。CD4、CD8、CD4/CD8、补体C3、IgG和IgM含量也明显增加(P＜0.01，P＜0.05)。结论：β-1,3-1,6葡聚糖对S180腹水移植瘤生长具有明显抑制作用，其机制可能是通过促进细胞免疫和体液免疫的功能而实现的。%Objective: To investigate the inhibition ofβ-1,3-1,6 glucan derived from black yeast on the growth of transplanted ascites tumors and immune functions in mice. Methods: BALB/c mouse models with transplantation of S180 ascites tumors were established. The BALB/c mice bearing S180 ascites tumors were intervened by oral administration of black yeastβ-1,3-1,6 glucan once daily. The volume of S180 ascites tumors was measured regularly during the 21-day administration period. Moreover, the levels of ten cytokines including IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IFN-γ, TNF-α, TNF-β and G-CSF in mouse serum were assayed and the contents of CD4, CD8, C3, IgG and IgM were also measured. Results: Compared with the blank control group, the intervention group showed a signifi cant increase in the levels of the ten cytokines (P<0.01, P<0.05). In addition, the levels of CD4, CD8, CD4/CD8 ratio, C3, IgG and IgM were also increased significantly (P<0.01, P<0.05). Conclusion:β-1,3-1,6 glucan derived from black yeast can considerably inhibit the transplanted tumor growth of S180 and the mechanism underlying this great inhibitory effect may be related to improved cellular and humoral immune functions.