响应面法优化鳕鱼皮胶原蛋白肽螯合铁工艺

摘要

The optimal chelating conditions for cod skin collagen peptide and ferrous chloride were investigated by response surface methodology（RSM） to explore the effects of pH（X1）,collagen peptide-ferrous chloride ratio（X2） and collagen peptide concentration（X3） on chelating efficiency.The optimal chelating conditions were collagen peptide-ferrous chloride ratio of 4：1,collagen peptide concentration of 3.5% and pH 7.0.Under the optimal chelating conditions,the predicted and experimental yields of the peptide-iron（Ⅱ） chelate were 37.46% and 37.31%,respectively.The infrared spectral analysis revealed that the ferrous ions were chelated with NH2＋and COO-groups,suggesting the formation of a new kind of peptide-iron（Ⅱ） chelate.%采用响应面法优化鳕鱼皮胶原蛋白肽与氯化亚铁进行螯合反应的条件,制备小分子肽螯合铁产品。以pH值、小分子肽与FeCl2的质量比和小分子肽液质量分数3因素的5水平进行二次正交旋转组合试验,建立螯合物得率的二次回归方程。结果表明：最佳螯合工艺条件为胶原蛋白肽与氯化亚铁的质量比4：1、小分子肽液质量分数3.5%、pH7.0。在此条件下,螯合物得率为37.31%,与模型的预测值37.46%接近。红外光谱检测结果显示,亚铁离子与小分子肽中的NH2＋和COO-有螯合,是一种新型螯合物。