研究体外消化前后蓝靛果提取物的总酚和花色苷含量、花色苷组成以及抗氧化能力的变化。实验分别采用福林-酚法和pH示差法测定总酚和花色苷含量，利用高效液相色谱-电喷雾二级质谱联用技术结合分子质量，离子碎片，出峰顺序及参考文献分析花色苷组成；采用总抗氧化能力（total antioxidant capacity，T-AOC）和氧自由基吸收能力（oxygen radical absorbance capacity，ORAC）法测定体外消化前后提取物的抗氧化能力。结果表明：模拟体外消化后样品总酚含量增加了48.2%，花色苷含量降低了67.6%，单体花色苷由11种降为8种，此外，ORAC值和T-AOC值分别下降了80.0%和55.6%。综上，与未经消化提取物相比较，模拟体外消化处理的蓝靛果提取物的多酚含量增加，花色苷含量和种类均减少，抗氧化能力下降。%Changes in total polyphenols and anthocyanins contents, anthocyanin composition and antioxidant capacity of Lonicera caerulea berry extracts before and afterin vitrodigestion were investigated. Folin-Ciocalteu and pH differential methods were used for determining the contents of total polyphenols and anthocyanins, respectively. Anthocyanin composition was analyzed using HPLC-EIS-MS/MS based on molecular mass and fragment ions, peak sequence and related literature data. Antioxidant activities of the extracts before and after in vitro digestion were measured using total antioxidant capacity (T-AOC) and oxygen radical absorbance capacity (ORAC) methods. The results indicated that the content of total polyphenols increased by 48.2% while anthocyanins decreased by 67.6%. The number of anthocyanins decreased from 11 to 8; additionally, the ORAC and T-AOC values of the extracts decreased by 80.0% and 55.6% afterin vitrodigestion, respectively. Taken together, afterin vitrodigestion,Lonicera caerulea berry extracts had increased total polyphenols, reduced anthocyanin contents and composition, and lowered antioxidant activity.
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