建立一种快速、高效测定芝麻油中苯并（a）芘的固相萃取-高效液相色谱（SPE-HPLC）方法。样品前处理采用硅胶固相萃取柱串联氧化铝固相萃取小柱，稀释液和淋洗液选择正己烷，洗脱液选择二氯甲烷，在40℃下氮吹干后，乙腈复溶后检测。采用Waters RP C18色谱柱（4.6 mm×250 mm，5μm）分离，以乙腈-水（90∶10）作为流动相，用荧光检测器检测，外标法峰面积定量。结果表明，苯并（a）芘在1.00 ng/mL~20.00 ng/mL浓度范围内线性良好，相关系数r2为0.9998，检出限为0.05μg/kg，定量限为0.16μg/kg，加标回收率达到92.2%~98.5%。%A rapid and effective method was established for the determination of benzo (a) pyrene in sesame oil by solid-phase extraction coupled with high performance liquid chromatography (SPE-HPLC). The samples were treated by silica gel SPE tandem alumina solid-phase extraction column,and the leaching solution of n-hexane,the eluent of dichloromethane. The elution liquid was dried at 40 ℃under nitrogen,and detected after re-dissolution with acetonitrile.The separation of targeted compound was performed on a Waters RP C18 chro-matographic column(4.6 mm×250 mm,5μm) using acetonitrile-water(90∶10) as mobile phase,with fluores-cence detector and external standard method peak area quantification. The linear range of benzo(a) pyrene was in the range of 1.00 ng/mL-20.00 ng/mL with a correlation coefficient of 0.999 8.The detection limit was 0.05μg/kg and the quantitative limit was 0.16μg/kg. The recovery rate was 92.2%-98.5%.
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