利用鱼糜漂洗水回收的蛋白肽制备肽钙复合物

摘要

Based on binding rate, the conditions ofrecycledmyogenpeptide (RMP) from surimi wash water binding calcium were optimized as follows:RMP concentration 5 g/L, calcium concentration 4 mmol/L, tem-perature 35℃, pH 9 and reaction time 1 h. In these conditions the binding rate of RMP was 85.00%. RMP-Ca complex owned good ability to resist pH with more than 85%calcium retention rate at the pH range of 5-9. Af-ter heated at 121.1℃for 15 min, the calcium retention rate was as high as 70.14%. RMP-Ca complex also could resist digestion by pepsin and trypsin with 95.31%calcium retention rate after digestion.%以钙结合率为指标，对从鱼糜漂洗废水中回收的肌浆蛋白肽（recycled myogenpeptide， RMP）与钙离子的结合条件进行优化。最佳结合条件为pH9、35℃、钙离子浓度4 mmol/L、RMP浓度5 g/L、反应时间1h，此时钙结合率为85.00%。RMP-Ca复合物具有较好的酸碱稳定性，在pH 5~9范围内钙保留率高达85%以上。一定的加热处理会导致RMP-Ca复合物钙保留率下降，经过121.1℃高温处理后钙还能保留70.14%。RMP-Ca复合物具有很好的抗消化性，消化后钙保留率高达95.31%。