山葡萄皮花青素超声波辅助提取及抗氧化活性研究

摘要

为了提高山葡萄皮的利用率,采用超声波辅助提取山葡萄皮花青素.在单因素试验的基础上,采用Box-Behnken设计方法和响应面法优化山葡萄皮花青素提取条件.结果表明:超声波辅助提取山葡萄皮花青素最优的工艺条件为:超声波功率580 W、超声温度48℃、乙醇浓度58 %、料液比1:29(g/mL).在此条件下,理论花青素得率为86.33 %,试验条件下花青素得率的平均值为83.17 %,理论值和试验值相对误差3.66 %.在最优工艺下测定花青素抗氧化活性,研究发现随花青素浓度的增加,对DPPH·和·OH的清除率显著增加且高于抗坏血酸(VC).研究结果对超声波提取花青素技术的研究具有较好的借鉴意义.%In order to improve the utilization rate of Vitis amurensis Rupr skin,ultrasonic assisted extraction was employed to extract anthocyanins fromVitis amurensis Rupr skin.On the basis of single factor experiment, the optimal extraction conditions were investigated by using the Box-Behnken design method and response surface method. The results showed that the optimal extraction processing parameters were: ultrasonic power 580 W,ultrasonic temperature 48℃,ethanol concentration 58 %,solid-liquid ratio 1:29(g/mL).Under the optimal condition,the extraction rate of theoretical anthocyanin was 86.33 %,and the average extraction rate of anthocyanin was 83.17 % under the experimental conditions,and the relative error between theoretical value and experimental value were 3.66 %. The antioxidant activity of anthocyanins was determined under the optimum conditions.It was found that the scavenging rate of DPPH·and·OH were significantly higher than that of ascorbic acid(VC)with the increase of anthocyanin concentration. The results of this study have great significance on research of ultrasonic extraction anthocyanins technology.