一株低温产纤维素酶细菌的筛选及其发酵产酶条件的优化

摘要

从不同土壤样品中分离得到一株低温产纤维素酶高活性的细菌，并对其液态发酵条件进行优化。结果表明，此菌株液体发酵培养基最佳配方为：蛋白胨15.0g，羧甲基纤维素钠（CMC-Na）10.0g，秸秆粉10.0g，氯化钠5.0g；最佳培养条件为：培养基pH为6.0，发酵时间为4d，发酵温度为20℃，接种量为4%，摇床转速为180r/min；发酵条件优化后发酵液中纤维素酶活（CMCA）为38.96U/mL，是优化前的2.2倍。%A low temperature strain bacteria producing cellulose was isolated from variety of soil samples , and the liquid fermentation conditions were optimized. The results of experients showed that the primum culture medium were consisted with peptone 15.0g, carboxymethyl cellulose sodium (CMC-Na)10.0g, straw powder 10.0g; optimal culture conditions were fermentation medium pH of 6.0, fermentation time of 4d, fermentation temperature 20℃, 4% inoculated quantity, table speed of 180r/min. Under the optimum conditions, the cellulase activity (CMCA) of the fermented liquid was 38.96U/mL, and was 2.2 times as before.