以巴旦木蛋白为底物，采用正交试验优化碱性蛋白酶水解巴旦木蛋白的条件，研究巴旦木蛋白肽的还原能力、对羟自由基、超氧阴离子自由基以及二苯代苦味酰基自由基的清除能力。结果表明：最优酶解工艺参数为底物浓度20％、酶浓度4％、pH值9．5、酶解温度60℃，在此条件下巴旦木蛋白的水解度为36．6％。巴旦木蛋白肽的还原能力随肽浓度的增大而增大，对OH·、O^2-·以及DPPH·均具有良好的清除能力，清除效果随着巴旦木蛋白肽浓度的增大而增强，两者之间存在着量效关系，但当肽达到一定浓度时，其抗氧化效果基本不受浓度影响。%With the badanmu protein as the substrate, the orthogonal test was taken to study the enzymatic hydrolysis condition of alcalase enzyme on badanmu proteins. The reducing power and the scavenging activity to hydroxyl, superoxide radical, and DPPH · free radicals were also investigated. The optimum hydrolysis parameters were as follows ： substrate concentration 20% , enzyme concentration 4% , pH value 9.5, temperature 60℃. In this condition of hydrolysis, its hydrolysis degree for badanmu protein is 36.6%. The reductive ability of badanmu protein peptide increased with peptide concentration increasing. The protein peptide also had good OH·, O2- , and DPPH · scavenging activity, which increased with the concentration of peptides until its peak. The antioxidation activity of badanmu peptides were almost fixed wheff its concentration exceeded a certain level.
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