乌鸡肽铁(Ⅱ)螫合物的制备及红外光谱鉴定

摘要

以乌鸡低聚肽为原料,FeCl2为铁源优化乌鸡肽铁(Ⅱ)螯合物的制备工艺,通过测定铁离子螯合率和多肽铁得率衡量螯合效果,并通过傅里叶红外光谱对螯合产物进行结构分析.优化结果表明,乌鸡肽铁(Ⅱ)螯合物的最佳螯合条件为:乌鸡肽浓度,4％ (g/mL)；乌鸡肽与亚铁盐的质量比,5:1,pH值,4.在此条件下乌鸡肽铁(Ⅱ)螯合物的螯合率为(84.76±0.12)％,螯合物得率为(40.27±0.15)％.红外光谱分析表明,乌鸡肽铁(Ⅱ)螯合物中Fe2+与NH2+以及-COO-形成配位键,说明成功生成了一种新型肽铁螯合物.%Silky fowl oligopeptides and FeCl2 were used as raw materials.The chelating ratio of iron and the yield of the peptide-iron (Ⅱ) chelate were measured to assess the chelating reaction.The structure of the chelate was analyzed by the method of Fourier infrared spectrum.The result of orthogonal experiment showed that the optimal condition of the chelating reaction was as follows:the concentration of black-bone silky fowl oligopeptides was 4％ ; the mass ratio of oligopeptides to FeC12 · 4H2O was 5∶ 1,and the pH value was 4.Under the optimal chelating conditions,the chelated iron ratio reached (84.76 ± 0.12)％,and the productive index of the peptide-iron (Ⅱ) chelate was (40.27-±0.15)％.The result of infrared spectral analysis revealed that the ferrous ions were chelated with NH2+ and COO-groups of black-bone silky fowl oligopeptides,suggesting the formation of a new kind of peptide-iron (Ⅱ) chelate.