ISAV实时荧光RT-PCR检测方法的建立

摘要

A real-time PCR method was developed for the specific,sensitive and rapid detection of ISAV(Infectious salmon anemia virus).A pair of specific primers and a specific probe was designed according to the ISAV gene conserved sequence.The real-time fluorescence PCR probe for detecting ISAV was established.The results showed that this method had good specificity and repeatability,the detection of ISAV minimum copy number was 13.Therefore,the study established the ISAV specific,sensitive and rapid real-time fluorescence PCR probe for fishery products.%为建立ISAV(传染性鲑鱼贫血症病毒)实时荧光定量PCR检测方法,针对ISAV基因序列保守区域设计一对特异性引物和一条特异性的探针,建立实时荧光RT-PCR探针检测法,并进行特异性、敏感性、重复性检测.结果表明:所建立的ISAV实时荧光RE-PCR探针检测法,引物和探针特异性良好,组内组间重复性良好,检出ISAV最小拷贝数为13拷贝.