液相色谱串联质谱法测定食品中红曲色素

摘要

In this paper,liquid chromatography-tandem mass spectrometry was used to determine monascorubramine,monascus red,monascin,ankaflavin of the residues in food,using selected ion monitoring for positive confirmation.Liquid sample is extracted with water by the ultrasonic processor.After centrifugation and volume,solid samples or hemi-solid samples were dissolved to a constant volume by water.Then by C t8 solid phase extraction cartridges,eluted sample was determined by LC-MS/MS using external standard method.The detection limits,linear ranges,and recoveries of this method,for monascorubramine,monascus red,monascin,ankaflavin,were 1.0 mg/kg,1.0 ～ 100.0 mg/kg and 89.3％ ～ 94.3％.This study established a method for determination of monascus pigment in foods.It is quickly and sensitive,and can simultaneously determine many kinds of monascus pigments and their positive confirmations.%采用液相色谱串联质谱法测定了食品中红曲红胺、红曲红素、红曲素、红曲黄素的含量,并选择离子检测进行阳性确证.液体试样用水超声提取,离心定容后,固体和半固体样品采用水溶解定容,提取液再经固相萃取柱净化,样液经洗脱定容后,供液相色谱-质谱/质谱仪测定和确证,外标法定量.该方法的最低检出限、线性范围和方法回收率分别为:1.0 mg/kg、1.0 ～ 100.0 mg/kg和89.3％～94.3％.