一种检测葡萄糖氧化酶活力的新方法

摘要

采用SBA-40C型生物传感分析仪建立了一种葡萄糖氧化酶活力的快速测定方法.利用生物传感分析仪检测葡萄糖质量浓度的工作原理,葡萄糖氧化酶专一性地与β-D-葡萄糖反应,产生的过氧化氢在过氧化氢电极表面上发生电子转移,内置电子元件将电信号转变为数字信号,用已知活性单位的葡萄糖氧化酶作为测定标准定标后,即可在仪器上直接测出待测样品的葡萄糖氧化酶活性单位.结果表明:利用生物传感分析仪测定葡萄糖氧化酶活力时,缓冲液最佳pH为6.5,测定时间20 s,操作周期小于60 s,连续10次测定RSD值为0.63％,0 ～ 100 U/mL的范围内线性良好,r=0.999 1.该方法专一性高、简便、快速、准确、重复性好,适用于样品数目较多的葡萄糖氧化酶活力的快速测定.%The purpose of our work was to establish a method for determination of glucose oxidase activity using SBA-40C biosensor analyzer.This novel method was based on the working principle of the SBA-40C biosensor analyzer to detect the contents of glucose.That is,glucose oxidase specifically reacted withβ-D-glucose and produced hydrogen peroxide,then electron transfer occurred on the surface of Peroxide hydrogen electrode,after that the built-in electronic components turned electrical signals into digital signals.A known unit of glucose oxidase was used as measurement standard,the glucose oxidase activity of the tested sample can be detected on the SBA-40C biosensor analyzer directly.Results showed that optimum reaction pH was 6.5,measurement time was 20s,and the operation period was less than 60 s.The RSD value of 10 consecutive was 0.63％,and 0 ～ 100 U/mL was within the scope of linear good,r =0.9991.The method is special,simple,rapid,accurate,and reproducible,which was suitable for determination of glucose oxidase activity with numerous samples.