影响细胞体外培养的因素及无菌环境防控策略

摘要

The technology of animal cell culture has a rapid development in the modern biological science. In recent years, this technique from a simple experimental operation has extended to the life sciences, bio-medicine and other research disciplines and production areas, and it has become a widely used technical means. Currently, many biotechnology researches are inseparable from the technology of cell culture. This paper introduces the basic operation of cell culture methods, then mainly introduces the main influencing factors of animal cells culture in vitro, and the strategies of prevention and control in the sterile cell laboratory.%动物细胞培养是现代生物科学中发展十分迅速的一种实验技术.近年来,动物细胞培养技术已从单纯的实验操作扩展到生命科学、生物医药学等多个学科的研究和生产领域,成为广泛采用的技术手段,许多生物技术科学研究都离不开细胞培养.从细胞培养的基本操作方法人手,介绍了影响动物细胞体外培养的主要因素,以及对细胞实验室无菌环境的防控策略.