固相萃取-高效液相色谱法测定武汉东湖水体中微囊藻毒素

摘要

Microcystins (MC-RR and MC-LR) in Donghu Lake were determined by high performance liquid ehromatograph ( HPLC) with solid phase extraction ( SPE). The optimum condition of determination by HPLC and the elution solutions for sample preparation were discussed. Determination limits of the method were 0. 0789μg/L and 0. 0234μg/L, and linear arranges were 0. 1 ~ 10.0mg/L and 0. 06 ~ 10. 0mg/L for MC - RR and MC - LR, respectively. Recoveries of microcystins were from 78. 4% to 97. 4% . The RSD of determination was lower than 6. 3% . The method offers traits of high sensitivity, speediness and exactness, and was applied to the determination of MC-RR and MC-LR in water samples with satisfactory results.%建立了固相萃取-高效液相色谱法(SPE - HPLC)测定东湖水样中微囊藻毒素(MC)的方法,考察了不同流动相、淋洗液和洗脱液对MC - RR和MC - LR测定的影响.结果表明,MC - RR和MC - LR的方法检出限分别为0.0789μg/L和0.0234μg/L,其线性定量范围分别为0.1～10.0mg/L和0.06～10.0mg/L；样品测定回收率为78.4％～97.4％,RSD小于6.3％.该法灵敏度高,快速准确,用于实际水样测定的结果令人满意.