In this paper, RT PCR analysis on SOX gene of seven tissues from the Chinese soft shelled turtle (Pelodiscus sinensis) was studied, and SOX gene fragments of expression from the testicle, brain, heart, kidney and spleen were cloned using RT PCR products.The results show that SOX genes has specific expression in the testicle, brain, spleen, cardiac muscle and kidney and isn’t expression in muscle, liver and ovary of femel.The results of sequence reveal that the SOX genes of expression in the testicle are TSSOX1、TSSOX4 TSSOX5 and TSSOX9, and those are TSSOX2 and TSSOX4 in the brain, and this is TSSOX4 in the spleen and heart tissue, and those are TSSOX2 and TSSOX3 in the kidney tissue.This suggests that the SOX gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiation of male germ cell.
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