葡萄皮渣中原花青素对HL-60细胞作用的研究

摘要

目的 探讨原花青素(GPC)在体外对人白血病细胞(HL-60细胞)增殖抑制及细胞凋亡的诱导作用.方法 体外培养HL-60细胞,分别用100、200、300、400、500 μg/mL GPC作用.MTT法检测不同浓度GPC作用24、48、72 h后HL-60细胞的增殖抑制率,流式细胞术检测24 h后HL-60细胞的凋亡情况.结果 随着GPC药物浓度的增加,其对HL-60细胞的增殖抑制率逐渐增高,呈时间-剂量依赖效应关系;GPC在低浓度(200 μg/mL)时可引起少量的细胞发生凋亡,随着GPC浓度的增加,细胞凋亡率也在不断增加,呈明显的量效关系.结论 GPC可在体外抑制HL-60细胞的增殖,并诱导HL-60细胞凋亡.%Objective To probe the effects of procyanidins inducing inhibition of proliferation and apoptosis in human leukemia cells(HL-60 cells) in vitro. Methods Human leukemia HL-60 cells were respectively cultured with different concentrations of pro-cyanidins(100,200,300,400 and 500 μg/mL). We detected the proliferation of HL-60 cells after 24 ,48 and 72 h by MTT methods. HL-60 cells were cultured with different concentrations of procyanidins for 24 h,and their apoptosis were determined through flow cytometry. Results The growth inhibitory rate of HL-60 cells increased along with the concentration of procyanidins and it had the concentration and time dependent;Treated by 200 μg/mL procyanidins for 24 h could induce apoptosis of HL-60 cells,the apoptosis rate of HL-60 cells increased along with the concentration of procyanidins in an apparent concentration-effect dependant manner. Conclusion Procyanidins can inhibit proliferation and induce apoptosis of HL-60 cells in vitro.