Th17细胞相关因子与寻常型银屑病的相关性研究

摘要

目的 通过对寻常性银屑病患者血清中白细胞介素(IL)-17、IL-23、IL-6及转化生长因子-β(TGF-β)水平的检测,探讨Th17细胞相关因子在银屑病发病机制中的作用.方法 选择30例寻常性银屑病患者(静止期组11例,进行期组19例)和10例健康体检者(对照组)为研究对象.采用亲和素-生物素过氧化物酶复合物酶联免疫吸附试验(ABC-ELISA)检测血清IL-17、IL-23、IL-6和TGF-β的水平.结果 银屑病患者进行期组和静止期组血清IL-23、IL-6水平均明显高于对照组(P＜0.05),进行期组血清TGF-β水平也明显高于对照组(P＜0.05),而血清IL-17水平与对照组差异无统计学意义(P>0.05),在两组银屑病患者间,血清IL-17、IL-23、IL-6和TGF-β水平差异均无统计学意义(P>0.05).银屑病患者血清IL-23水平与严重程度指数(PASI)评分呈正相关(r=0.424,P<0.05 );而血清IL-17、IL-6、TGF-β与PASI均无相关性(r=-0.118、r=-0.043、r=-0.203,P>0.05).结论 寻常型银屑病患者血清IL-23、IL-6和TGF-β水平升高,且IL-23水平与PASI呈正相关,Th17细胞可能参与了银屑病的发病过程,但与银屑病病情分期无关.%Objective To explore the roles of Thl7 related cytokines in the pathogenesis of psoriasis by detecting the serum levels of IL-17 ,IL-23 ,IL-6 and TGF-β in the patients with psoriasis vulgaris .Methods 30 cases of psoriasis vulgaris were divided into the developing stage group(19 cases) and the resting stage group(11 cases) ,with 10 healthy persons as the control group .The serum levels of IL-17 ,IL-23 ,L-6 and TGF-β were detected by avidin-biotin peroxidase complex enzyme-linked immunosorbent assay (ABC-ELISA) .Results The serum IL-23 and IL-6 levels in the developing stage and the resting stage group were significantly higher than those in the control group (P<0 .05 ) .The serum TGF-β level in the developing stage group was significantly higher than that in the control group(P<0 .05) .there was no statistical difference in the serum IL-17 level between the two groups (P> 0.05).There were no statistical difference in the serum IL-17 ,IL-23 ,IL-6 and TGF-β levels between these two psoriasis groups (P>0 .05) .There was positive correlation between the serum IL-23 level and the Psoriasis Area and Severity Index (PASI) scores in psoriasis patients (r=0 .424 ,P<0 .05), while there was no correlation between the serum IL-17 ,IL-6 ,TGF-β levels and PASI scores (r= -0 .118 ,r= -0 .043 , r= -0 .203 ; P>0 .05 respectively) .Conclusion The serum IL-23 ,IL-6 and TGF-β levels are increased in the patients with psoriasis vulgaris .The serum IL-23 level is positively correlated with the PASI scores .Th17cells may participate in the pathogenesis of psoriasis, but has no correlation with the stages of psoriasis.