目的：探讨微小RNA‐34a（miR‐34a）在碘乙酸钠所致大鼠膝骨关节炎模型中的表达变化，并初步明确其功能。方法选取30只雄性Wistar大鼠，左侧膝盖不注射碘乙酸钠作为对照组，右侧膝盖注射碘乙酸钠作为模型组；造模完成后检测两组大鼠关节液内miR‐34a表达水平的变化；分离两组大鼠的膝关节软骨细胞并进行原代培养，敲低miR‐34a后，使用流式细胞仪测定细胞凋亡情况。结果模型组M akin评分为（5．09±1．35）分，对照组为（1．27±0．64）分，差异有统计学意义（ P＜0．05）。与对照组比较，模型组中miR‐34a在关节液和关节软骨细胞内的表达均升高，差异均有统计学意义（P＜0．05）。将miR‐34a用它的反义互补片段敲低后，模型组大鼠关节软骨细胞的凋亡受到明显抑制（P＜0．05）。结论 miR‐34a在碘乙酸钠所致大鼠膝骨关节炎的关节液中表达升高，并且抑制miR‐34a的表达会降低关节炎大鼠的关节软骨细胞凋亡率。%Objective To explore the expression of microRNA‐34a(miR‐34a) in sodium iodoacetate‐induced rat model of os‐teoarthritis rat model and to initially clarify its function .Methods Thirty male Wistar rats were selected .The right knees were in‐jected with sodium iodoacetate (model group) and the left knees served as the control group without injecting by sodium iodoace‐tate .The expression levels of miR‐34a in the synovial fluid and articular chondrocytes were compared between two groups after con‐structing the model .Articular chondrocytes in the two groups were separated for conducting the primary culture ,after miR‐34a knockdown ,the flow cytometry was used to determine cell apoptosis .Results The Makin score in the model group was(5 .09 ± 1 .35)points ,which was (1 .27 ± 0 .64)points in the control group ,the difference had statistical significance (P<0 .05) .Compared with the control group ,the expression levels of miR‐34a in the synovial fluid and articular chondrocytes in the model group were in‐creased ,the difference was statistically significant(P<0 .05) .After the miR‐34a knockdown by using its complementary antisense fragment ,articular chondrocytes apoptosis in the model group was significantly inhibited (P<0 .05) .Conclusion The expression of miR‐34a in the synovial fluid of sodium iodoacetate‐induced knee osteoarthritis is increased ,moreover inhibiting the microRNA‐34a expression could reduce the apoptotic rate of articular chondrocytes in osteoarthritis rat .
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