AIM: To develop a rapid high-performance liquid chromatographic method for the determination of bisbibenzyls in four bryophytes. The effects of species and sample locations on the bisbibenzyi composition and con-tent were also evaluated. METHOD: Chromatographic separation was achieved on a Hypersil C18 column (150 mm×4.6 mm, I.D. 5μ m) with acetonitrile-water gradient elution (each containing 0.05% formic acid). UV detection was per-formed at 280 nm. RESULTS: A total of 6 bisbibenzyls were detected in four bryophytes, and the results show that the sample locations and species have an influence on the bisbibenzyl composition and the content of each bisbibenzyl. CONCLUSION: This method not only provides a useful tool for analyzing and quantifying the bisbibenzyls but also provides useful information on the phytochemical research of these bryophytes.%目的:建立一种反相高效液相色谱法以快速分析四种苔藓植物中双联苄类化合物,并对不同种属不同采集地点的苔藓植物中双联苄类化舍物进行分析比较.以考察种属及采集地点对该类化合物的种类和含量影响.方法:采用Hypersil C18柱(150mm×4.6 mm,I.D.5 μm)分离,以乙腈-水(各含0.05%酸)为流动相,线性梯度洗脱,于280 nm波长下检测.结果:共检测出6种双联苄类化合物,并且种属和采集地点差异对该类化合物的种类和含量有不同程度的影响.结论:建立了一种有效的分析及测定不同苔类植物中双联苄类化合物的方法,并可为苔藓植物化学成分研究提供参考信息.
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