重组金黄色葡萄球菌肠毒素A协同增强破伤风类毒素的免疫原性研究

摘要

目的 重组超抗原金黄色葡萄球菌肠毒素A (SEA)与Al(OH)3佐剂联合应用协同增强破伤风类毒素(TT)的免疫原性观察.方法 将高、低两个剂量的TT分别与高、中、低3个剂量的重组SEA同时吸附一定浓度的Al(OH)3作为样品组,同时设置高、低剂量TT分别与同样浓度Al(OH)3的吸附组作为对照；各组分别腹部皮下免疫NIH小鼠8只,0.5 ml/只；免疫4周后,摘眼球采血,分离血清,检测各组抗TT抗体的IgG水平.结果 在TT高剂量组,高、中剂量的SEA均能增强抗TT抗体的IgG水平(P＜0.05),而低剂量SEA组与对照组相比未能增强TT的免疫原性；在TT低剂量组,3个剂量的SEA均能显著增强TT的免疫原性,P＜0.05.结论 TT联合重组SEA及Al(OH)3佐剂能诱导免疫后小鼠产生更强的体液免疫应答,SEA及Al(OH)3佐剂对TT具有协同免疫增强效应.%Objective To observe the immune effect of tetanus toxoid (TT) combined with staphylococcal enterotoxin A and aluminum hydroxide adjuvant in mice.Methods The high dose and low dose TT were mixed with three different doses (high,middle and low) of recombinant SEA respectively,and each group was adsorbed to the same concentration of aluminum hydroxide adjuvant as test group.At the same time,two different doses of TT adsorbed with aluminum hydroxide adjuvant as control.NIH mice were divided into eight groups randomly,and eight mice in each group.The each group mice were intraperitoneally injected with 0.5 ml sample respectively.The mice were killed four weeks later,of which the sera were separated and used to detect the IgG levels of anti-TT antibodies.Results Both high and middle doses of SEA adsorbed with high dose TT increased the IgG levels of anti-TT antibodies (P ＜ 0.05),while the low dose of SEA could not enhance the immunogenicity of TT compared with control group.In each group of low dose TT,the three different doses of SEA raised the IgG levels of anti-TT antibodies (P ＜ 0.05).Conclusion TT combined with SEA and aluminum hydroxide induced strong humoral immune responses in mice as compared with control,and SEA and aluminum hydroxide adjuvant show synergistic effects with TT.