小肠结肠炎耶尔森菌在达乌尔黄鼠鼠疫疫源地分布及特征研究

摘要

目的 了解小肠结肠炎耶尔森菌在达乌尔黄鼠鼠疫疫源地动物中的种间带菌分布情况及其特征,为我国小肠结肠炎耶尔森菌的研究提供科学的参考数据.方法 在达乌尔黄鼠鼠疫疫源地内选择牧区、半农半牧区和农区3个不同生态环境,分别采集鼠类、家畜家禽动物的粪便、舌根、咽拭子及肠道内容物等样本,进行小肠结肠炎耶尔森菌的分离、鉴定和毒力基因检测.结果 共检测各类样本3 260份,检出小肠结肠炎耶尔森菌65株,总检出率为1.99％;猪的带菌率最高,其携带O∶3/3、O∶5/1A、O∶4/4 3个血清生物型;其中O∶3/3型致病性菌株携带ail、ystA、yadA、VirF、rfbc毒力基因;且在牧区、半农半牧区、农区的样本均可检出阳性菌,以农区最高,检出率为9.95％.结论 小肠结肠炎耶尔森菌在内蒙古达乌尔黄鼠鼠疫疫源地中分布较广,以农区为重;猪是致病性小肠结肠炎耶尔森菌的主要携带者.%In order to investigate the distribution of Yersinia enterocolitica in Citellus dauricus plague focuses in Inner Mongolia,three different ecological environ/ments were chosen as the sampling area.Feces,tongue roots throat swabs,and intestinal contents of rodent,livestock,and poultry were separately collected,and different Y.enterocolitica strains were isolated,and identified.PCR analysis was conducted to detect the toxicity genes of Y.enterocolitica.Statiscal analysis was performed by chisquare test.Of the 3 260 samples,65 Y.enterocolitica strains were isolated and the overall detection rate was 1.99％.To include O ∶ 3/3,O ∶ 5/1A,O ∶ 4/4 serum biological type,the pathogenic strain of serotype O ∶ 3 and biological typt 3 carryinq toxicity genes ail,ystA,VirF yadA and rfbc was isolated from pigs in Citellus dauricus plague focuses,Inner Mongolia are the major carrier of pathogenic Y.enterocolitica distributed in three different ecological environment,and distributed mainly in agricultural area.