将琯溪蜜柚和度尾蜜柚2个品种的雌蕊,分别短截成柱头、花柱和子房3个部位的组织块,移植到培养基上培养,播上本品种或异品种的花粉,通过荧光显微技术,观察雌蕊的不同部位对交配亲和性的影响.结果表明:本品种或异品种的花粉虽然在3个部位组织块上均能正常萌发,但在本品种子房组织块上出现了强烈不亲和反应:琯溪蜜柚在胚囊中观察到因胼胝质积累而出现的强烈荧光.推测子房是2个品种自交不亲和的主要抑制部位.胚囊也是琯溪蜜柚不亲和的关键抑制部位.%The pistils of Citrus grandis (L.) Osbeck ' Guanximiyou' and " Duweimiyou' were cut into 3 tissue parts,stigma,style and ovary,which were then transferred onto culture medium and pollinated with pollens from both the same cultivars and different cultivars to detect the reaction of different pistil parts on the cross compatibility by fluorescence microscopy. The results showed that pollens either from the same cultivars or different cultivars were able to germinate normally on all the 3 tissue parts,but strong incompatible reaction occurred on ovary tissue part from the same cultivars,and that strong fluorescence due to accumulation of callose was detected from the embryo sacs of ' Guanximiyou'. Therefore,it was supposed that ovary was the main inhibitory part for self-incompatible reaction of the 2 cultivars,and that embryo sac was also the primary inhibitory part for self incompatible reaction of 'Guanximiyou'.
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