杨梅花色苷分离纯化制备矢车菊素-3-葡萄糖苷的研究

摘要

Cyanidin-3-glucosid was separated and purified successfully from waxberry anthocyanins by macroporous resin and Sephadex LH20 with 95％ (V/V) ethanol under pH3.0 and 0.5％ (V/V) HCl methanol solution as the eluents,respectively.The contents of cyanidin-3-glucoside of the products which were purified by macroporous resin and Sephadex LH20 were 29.05％ and 90.35％,respectively,compared with standard substance by HPLC.The product after purification is expected to replace imported standard for qualitative and quantitative analysis.%以杨梅汁为原料,分别选用95％pH3.0的盐酸乙醇和含体积分数为0.5％ HC1的甲醇溶液作为大孔树脂和葡聚糖凝胶的洗脱液,分离制备矢车菊素-3-葡萄糖苷.通过HPLC检测和标准品对照,经大孔树脂和葡聚糖凝胶纯化后,其纯度分别为29.05％和90.35％.