壳聚糖/羟基磷灰石骨复合支架修复关节软骨损伤

摘要

BACKGROUND:Studies have shown that the chitosan/hydroxyapatite composite scaffold with good biological performance is very similar to natural bone in structure, and plays the performance of different materials. OBJECTIVE:To observe the repairing effect of chitosan/hydroxyapatite composite scaffold on articular cartilage injury. METHODS:Thirty New Zealand white rabbits were selected to make articular cartilage injury on the left knee, and then model rabbits were randomly divided into sodium hyaluronate gel group (control group, n=15) and chitosan/hydroxyapatite composite scaffold group (experimental group, n=15). At 4, 8, 12 weeks after scaffoldimplantation, cartilage tissues were taken out and observed histological y. RESULTS AND CONCLUSION:Compared with the control group, the experimental group showed higher Wakitani scores at 4, 8, 12 weeks, but lower Mankin scores at 4 and 12 weeks after implantation (both P<0.05). Safranin-O staining results showed that at 4 weeks after implantation, chondrocytes arranged relatively in disorder with overt pleated smal projections in the control group, while chondrocytes were wel aligned with slightly pleated smal projections and slightly large nuclei in the experimental group;at 8 weeks, chondrocytes arranged irregularly with pleated smal projections in the control group, while in the experimental group, the chondrocytes arranged orderly with pleated smal projections and large nuclei;at 12 weeks, the chondrocytes in the control group exhibited a substantial y ordered arrangement with pleated smal projections and large nuclei, while in the experimental group, the chondrocytes arranged in order with no pleated smal projections but with large nuclei. Al these findings indicate that chitosan/hydroxyapatite composite scaffold can promote articular cartilage repair.%背景：壳聚糖/羟基磷灰石复合支架在结构上与天然骨十分类似，具有良好的生物学性能，能够发挥不同材料的性能。目的：观察壳聚糖/羟基磷灰石复合支架修复关节软骨损伤的效果。方法：取30只新西兰大白兔，制作左侧膝关节软骨缺损模型，随机分2组，实验组与软骨缺损处置入壳聚糖/羟基磷灰石复合支架，对照组置入透明质酸钠凝胶。置入后4，8，12周，取软骨损伤组织进行组织学观察。结果与结论：①组织学Wakitani与Mankin评分：实验组置入后4，8，12周Wakitani评分显著高于对照组(P<0.05)，置入4，12周的Mankin评分显著低于对照组(P<0.05)；②番红-O染色：置入后4周，对照组软骨细胞表面存在明显的褶皱状小突起，细胞相对不规则；实验组软骨细胞表面存在轻微的褶皱状小突起，细胞核略大，排列相对有序。置入后8周，对照组软骨细胞表面存在褶皱状小突起，细胞排列欠规则；实验组软骨细胞表面存在褶皱状小突起，细胞核较大，排列规则；置入后12周，对照组软骨细胞表面存在褶皱状小突起，细胞核变大，排列基本有序；实验组软骨细胞表面无褶皱状小突起；细胞核变大，排列规则；③结果表明：壳聚糖/羟基磷灰石复合支架可促进关节软骨损伤的修复。