新型携载人胰岛素样生长因子-1纤维屏障膜的制备及其理化和生物性能

摘要

通过同轴静电纺丝技术制备携载人胰岛素样生长因子-1(IGF-1)的纤维屏障膜(IGF-1/SF/PLLACL),其结构和性能经SEM,TEM和XRD表征.与非载药同轴静电纺丝纤维膜在表观形貌、理化性能、生物学性能和膜屏障功能等方面进行对比,研究了其在引导性骨组织再生中的可应用性.结果显示:纤维膜呈网状结构,IGF-1/SF/PLLACL形成芯壳双层结构;非载药纤维膜的接触角和机械强度均略高于载药组纤维,差异具有统计学意义(P＜0.05);载药组与非载药组纤维膜的衍射趋势与聚左旋乳酸聚己内酯晶体相似,丝素蛋白和胰岛素样生长因子-1的加入并未改变其相组成.3d内IGF-1突释现象严重,之后曲线逐渐平缓,呈缓释状态;载药组细胞活性和碱性磷酸酶活性较非载药组高,略低于IGF-1对照组,差异有统计学意义(P＜0.05),而且两组纤维膜均具有阻挡成纤维细胞长入的屏障功能.%The fibrous barrier membrane(IGF-1/SF/PLLACL) with the human insulin-like growth factor-1 (IGF-Ⅰ) were synthesized by coaxial electrospinning.The structures and properties were characterized by SEM,TEM and XRD.To evaluate its applicability in guided bone regeneration,drug loaded membrane was compared with the non-drug loaded coaxial electrospun fiber membrane in the aspect of morphology,physicochemical properties,biological properties and barrier function.The results showed that the fibrous membrane had reticular structure and IGF-1/SF/PLLACL formed a core-shell double layer structure.The contact angle and mechanical strength of the non-drug loaded fiber membrane was slightly higher than those of the drug loaded group,and the difference was statistically significant(P ＜0.05).The X-ray diffraction trend of fibrous membrane in drug-loaded group and non-drug-loaded group was similar to that of PLLACL crystal,and the addition of silk fibroin and insulin-like growth factor-1 did not change their phase composition.The burst release of IGF-1 was severe in 3 days,then the curve was gradual and tuned sustained release.The cell activity and alkaline phosphatase in the drug loaded group was much higher than that of the non-drug-loaded group and the IGF-1 control group (P ＜ 0.05),and both of the membranes had the barrier function to block the growth of fibroblasts.