条斑紫菜多糖对糖尿病大鼠脑组织的保护作用

摘要

目的 观察条斑紫菜多糖(PYP)对糖尿病大鼠脑组织的保护作用及对细胞凋亡的影响.方法 将60只Wistar大鼠随机分为:正常对照组、模型组、PYP高、中、低剂量组.HE染色观察脑组织病理变化,琼脂糖凝胶电泳法检测细胞凋亡的情况,RT PCR观察p53、bcl-2基因mRNA的表达.结果 PYP可以明显改善糖尿病大鼠脑组织病理形态改变.与模型组相比,PYP组大鼠脑组织凋亡细胞显著减少,p53基因表达明显减少,bcl-2基因表达明显增加.结论 PYP可以抑制糖尿病大鼠脑组织的p53基因表达,促进bcl-2基因表达,降低脑损伤.%Objective To observe the effect of porphyra yezoensis polysaccharide (PYP) on cerebrum tissue of diabetic rats and apoptosis. Methods 60 Wistar rats were randomly divided into normal control group, diabetic model group, and treated groups respectively with PYP (0.25 g/kg · d), PYP (0.5 g/kg · d), and PYP (1 g/kg · d). The cerebrum pathologic changes were observed by HE staining method, and the number of apoptotic cells was observed by electrophoresis and the mRNA expression of p53 and bc1-2 was confirmed by RT-PCR. Results PYP improved the cerebrum pathologic changes of diabetic rats. With comparison to the diabetic model group, the cerebrum pathologic change in the treated groups also improved. And the number of apoptotic cells, the expression of p53 in cerebrum tissue decreased and expressions of bc1-2 rose. Conclusion PYP can decrease the expression of p53 in cerebrum tissue and rise expressions of bc1-2 so as to protect brain tissues from cerebral ischemia.