六味地黄丸对绝经期肾阴虚骨质疏松症的基因表达调控数据分析

摘要

Objective To reveal the molecular mechanism of rehmanniae bolus on postmenopausal osteoporosis with kidney-Yin deficiency by analyzing the gene expression database.Methods The bioinformatics analysis tools QOE,DAVID,and STRING were used for analysis of the data related to the treatment of postmenopausal osteoporosis with rehmanniae bolus in GEO database of NCBI.Results Through a comparison of the gene expression profiles with patients before and after treatment with rehmanniae bolus,and an analysis of the function enrichment and protein-protein interaction of the differentially expressed genes,we showed that the treatment with rehmanniae bolus on postmenopausal osteoporosis with kidney-Yin deficiency increased the expression of the genes TNP1 and MAP3K10,and reduced the expression of most of the ZNF family genes and other genes such as XRN1,XPO1 and NUP54.Further data showed that down-regulation of XRN1 gene and ZNF family genes decreased the inhibition of the expression of estrogen,and up-regulated TNP1 expression enhanced the binding of estrogen and estrogen receptor.Meanwhile the up-regulation of MAP3K10 expression and down-regulation of XPO1 and NUP54 gene expression suppressed the overreacted immune response.Conclusion The effect of rehmanniae bolus on postmenopausal of osteoporosis with Kidney-Yin Deficiency is probably through the increasing the expression of estrogen,improving the interaction of estrogen with estrogen receptor,and rebuilding the immune homoeostasis.%目的 利用生物信息学分析工具,从基因调控的角度阐述六味地黄丸治疗绝经期肾阴虚骨质疏松症的分子机制.方法 运用生物信息学分析工具QOE、DAVID和STRING,对美国国家生物信息中心(National Center for Biotechnology Information,NCBI)中的GEO数据库中六味地黄丸治疗绝经期肾阴虚骨质疏松症的相关数据进行挖掘分析.结果 生物信息学分析显示中药六味地黄丸在治疗绝经期肾阴虚骨质疏松症的过程中基因TNP1和MAP3K10的表达显著增加,而ZNF家族基因和XRN1、XPO1、NUP54等基因的表达显著下降.这些差异基因的功能富集和相关作用机理分析显示,下调XRN1基因和ZNF家族基因的表达可以降低其对雌激素表达的抑制;上调TNP1基因的表达促进雌激素与雌激素受体结合;上调MAP3K10基因的表达并下调XPO1基因和NUP54基因的表达能降低免疫应答.结论 六味地黄丸对绝经期肾阴虚骨质疏松症的有益影响主要是通过促进雌激素表达及与其受体结合,并平衡免疫应答而促进骨组织的形成,从而达到改善骨质疏松.