氟曲马唑对足癣分离病原菌的体外抗菌活性研究

摘要

Objective To evaluate the in vitro activities of flutrimazole against fungal pathogens isolated from tinea pedis.Meth?ods In vitro drug sensitivities were determined using the broth microdilution methods according to the CLSI M38?A2 ( for dermato?phytes) and M27?A3 (for yeasts) documents.Results When against Trichophyton rubrum,the range of minimum inhibitory concen?tration (MIC),MIC50,MIC90,and geometric mean (GM) MIC for flutrimazole were 0.031~2 μg/mL,0.5 μg/mL,1 μg/mL and 0. 637μg/mL;for bifonazole were 0.031~16 μg/mL,0.25 μg/mL,2 μg/mL and 0.634 μg/mL;no significant ( P=0.974) difference was found for the GM MIC between the two drugs.When against Trichophyton interdigitale,the range of MIC,MIC50,MIC90,and GM MIC for flutrimazole were 0.031~1 μg/mL,0.031 μg/mL,0.5 μg/mL and 0.17 μg/mL;for bifonazole were 0.125~16 μg/mL,1μg/mL,2 μg/mL and 1.886 μg/mL;significant (P=0.001) difference was found for the GM MIC between the two drugs.Even though there are a few number of yeast strains,this study showed that the two drugs had significant difference for the GM MIC against Candida spp.(P=0.000) and Trichosporon spp.(P=0.031).Conclusion In vitro activity of flutrimazole was similar to bifonazole when against T.rubrum,but better than bifonazole when against T.interdigitale,Candida spp.and Trichosporon spp..%目的 评价氟曲马唑对足癣分离病原菌的体外抗菌活性,并与联苯苄唑相比. 方法 采用CLSI推荐的M?38 A2 (皮肤癣菌)和M27?A3 (酵母菌)微量液基稀释法对病原菌进行体外药物敏感性测定. 结果 氟曲马唑对红色毛癣菌最小抑菌浓度 (MIC)范围为0.031~2 μg/mL,MIC50为0.5 μg/mL,MIC90为1 μg/mL,GM值为0.637 μg/mL;联苯苄唑分别为0. 031~16 μg/mL,0.25 μg/mL,2μg/mL,0.634μg/mL;两药对红色毛癣菌MIC GM值比较差异无统计学意义( P=0.974). 氟曲马唑对趾 (指)间毛癣菌MIC范围为0.031~1μg/mL,MIC50为0.031μg/mL,MIC90为0.5μg/mL,GM值为0.17μg/mL;联苯苄唑分别为0.125~16 μg/mL,1μg/mL,2μg/mL,1.886μg/mL;两药对趾(指)间毛癣菌MIC GM值比较差异具有统计学意义 ( P=0.001). 尽管酵母菌菌株数偏少,但研究结果显示两药对念珠菌属和毛孢子菌属MIC GM值比较差异具有统计学意义 (P=0.000和P=0.031). 结论 氟曲马唑对红色毛癣菌的抗菌活性与联苯苄唑相似,但对趾 (指)间毛癣菌、念珠菌属和毛孢子菌属的抗菌活性明显优于联苯苄唑.