血清及粪便髓过氧化物酶测定对溃疡性结肠炎患者辅助诊断与疾病活动性监测中的应用价值

摘要

目的 探讨血清及粪便髓过氧化物酶对溃疡性结肠炎患者辅助诊断及其监测疾病活动性的应用价值.方法 选取2010年1月到2012年4月就诊于北京大学第一医院消化内科的溃疡性结肠炎患者45例(男30例,女15例),根据Mayo评分系统将UC患者分为缓解期组(18例)和活动期组(27例).对照组25例(男15例,女10例).搜集两组外周血及粪便标本,采用ELISA法检测血清及粪便中髓过氧化物酶含量.两组间比较采用Mann-whitney U检验,双侧P<0.05为差异有统计学意义.结果 UC组与对照组中血清MPO(U/L)分别为[113.78(71.31-174.53)vs.62.66(43.39-113.39)],Z=-2.488,P=0.013;活动期与非活动期UC患者血清MPO分别为[102.67(70.52-176.70)vs.116.91(68.16-174.04)],Z=-0.255,P=0.799.UC组与对照组粪便MPO分别为[926.47(495.34-1264.07)vs.557.86(377.84-930.43)],Z=-2.507,P=0.012;活动期与非活动期UC患者粪便MPO分别为[1231.24(725.42-1550.77)vs.692.58(285.98-886.17)],Z=-3.197,P=0.001.结论 血清及粪便 MPO 检测对 UC 的辅助诊断有一定应用价值,粪便 MPO 同时可用于 UC 患者的疾病活动性评估.%Objective To investigate the value of serum and feces myeloperoxidase(MPO)in assist diagnosis and dis-ease activity of ulcerative colitis (UC).Methods 45 patients with UC and 25 control subjects were enrolled in present study.UC patients were divide into active(n=27)and inactive (n=18)group according to Mayo score.MPO was de-tected by ELISA.Mann-whitney U test was used for comparison of different groups.Results serum MPO between UC group and controls were [113.78(71.31-174.53)vs.62.66(43.39-113.39)],Z =-2.488,P =0.013,respectively;ser-um MPO between active and inactive group were[102.67(70.52-176.70)vs.116.91(68.16-174.04)],Z =-0.255,P=0.799,respectively.Fecal MPO between UC group and controls were[926.47(495.34-1264.07)vs.557.86 (377.84-930.43)],Z =-2.507,P =0.012,respectively;fecal MPO between active and inactive group were[1231.24(725.42-1550.77)vs.692.58(285.98-886.17)],Z =-3.197,P =0.001,respectively.Conclusion Serum and feces MPO was useful for auxiliary diagnosis of UC,feces MPO was a promising biomarker in monitoring disease activity of UC.