针刺激活下丘脑室旁核促肾上腺皮质激素释放激素相关神经元特异性研究

摘要

目的 观察针刺调节下丘脑-垂体-肾上腺皮质轴(HPA)功能的穴位特异性,并探讨针刺对HPA轴的调节是否具有累积效应.方法 实验用SD雄性成年大鼠.胞外记录下丘脑室旁核促肾上腺皮质激素释放激素相关神经元电活动,比较针刺同一经脉不同神经节段、相同或相邻神经节段不同经脉的20个穴位对该神经元的激活效应及强度的差异,并观察不同针刺次数对不同时间点外周血皮质酮水平的影响.结果 针刺20个穴位对该神经元的激活效应由大到小的顺序:肾俞>期门>肝俞>大椎>章门>腹哀>太乙>郄门>水沟>商曲>腹通谷>足三里>太冲>内关>京门>阳陵泉>承山>筑宾>三阴交>膻中.结论 与肾上腺位于相同或相邻神经节段的膀胱经、肝经的穴位具有特异性效应,且有累积效应.%Objective To observe the specificity of acupoints in regulation of hypothalamic pituitary adrenal (HPA) axis, and study the cumulative effect of acupuncture on HPA axis. Methods Experiments were performed on Sprague-Dawley male rats. Extracellular record the electric activities on corticotropin releasing hormone (CRH)-relative neurons in paraventricular area of hypothalamus, and compare the effects of 20 point acupuncture for same channel but different nerve segment, same or adjacent nerve segment but different channel. The changes of peripheral blood corticosterone level in different time and different acupuncture times were determined. Results The effects from large to small in 20 points was BL23>LR14>BL18>GV14>LR13>SP16>ST23>PC4>GV26>KI17>KI20>ST36>LR3>PC6>GB25> GB34>BL57>KI9>SP6>CV17. Conclusion The specificity of acupoints on effect of acupuncture regulation of HPA axis was bladder and liver channel points, which are in the same or adjacent nerve segment pertaining to the adrenal glands, and has cumulative effect.