Objective To observe the effects of Chinese medicinal herbs with the efficacy of invigorating spleen, upbearing the clear, and tonifying spleen on the obesity degree, fat hormones, and insulin resistance in diet-induced obesity (DIO) rats;To choose better anti-obesity herbs from different Chinese medicinal herbs that can tonify spleen. Methods Among the 130 Wistar rats, 10 were chosen as the blank control group (fed with basal forage), and the remaining 120 were administered with high-fat high-nutrition forage for 13 weeks. According to weight, 50 DIO rats and 10 diet-induced obesity resistance (DIO-R) rats were obtained. DIO rats were divided into model group (normal saline), sibutramine group, invigorating spleen group (Atractylodix Rhizome and Magnoliae Officinalis Cortex), upbearing the clear group (Bupleuri Radix and Aurantii Fructus Immaturus) and medicine for tonifying qi and spleen group (Astragali Radix). All groups received gavage with corresponding drugs. Rats in the blank control group and the DIO-R group received gavage with normal saline. The basal forage was administered to rats in the blank control group, while high-fat forage was continually given to rats in the other six groups. Insulin resistance index (IRI), blood glucose, triglycerides, cholesterol, tumor necrosis factor α (TNF-α) and adiponectin were detected after blood withdrawing. TNF-α and adiponectin in the fat homogenate were examined. Results Compared with the blank control group, body weight, IRI, and cholesterol of rats in the model group significantly increased (P<0.01);adiponectin in homogenate reduced (P<0.01);serum and adipose homogenate TNF-α increased (P<0.01). Compared with model group, body weight, IRI, cholesterol of rats in DIO-R group significantly decreased (P<0.01);adiponectin in fat homogenate increased (P<0.01). Body weight and cholesterol of rats in the sibutramine group significantly decreased (P<0.01), while serum and adipose homogenates TNF-αdecreased (P<0.05, P<0.01). Body weight, IRI and cholesterol of rats in upbearing the clear group decreased (P<0.05, P<0.01);serum and adipose homogenate TNF-α decreased (P<0.05, P<0.01);adiponectin increased in fat homogenate (P<0.05). IRI, cholesterol and serum TNF-αof rats in the invigorating spleen group decreased (P<0.05, P<0.01);adiponectin in serum and adipose homogenate increased (P<0.05). BMI, blood glucose, IRI and cholesterol of rats in Astragali Radix group decreased (P<0.05, P<0.01);TNF-α decreased and adiponectin increased in serum and adipose homogenate (P<0.05, P<0.01). Conclusion Astragali Radix could reduce obesity induced by high-fat forage, and its effects on improving glucose and lipid metabolism disorder and IR are better than the other TCM groups. Its mechanism is related to decreasing TNF-α and increasing adiponectin level.%目的：观察运脾、升清、补脾中药对饮食诱导肥胖(DIO)大鼠肥胖程度及脂肪激素、胰岛素抵抗(IR)的影响,从不同健脾中药中进一步筛选抗肥胖药物。方法 Wistar大鼠130只,10只作为空白组,给予基础饲料,其余120只给予高脂饲料13周喂饲,按体质量得到DIO大鼠50只和饮食诱导肥胖抵抗(DIO-R)大鼠10只,DIO大鼠分为模型组、西布曲明组、运脾组、升清组、黄芪组,每日分别给予生理盐水、西布曲明、运脾药(苍术和厚朴)、升清药(柴胡和枳实)、补气健脾药(黄芪)灌胃,空白组与DIO-R组予生理盐水灌胃。灌胃期间空白组予基础饲料,余6组继续予高脂饲料喂饲。取血测定胰岛素抵抗指数(IRI)、血糖、三酰甘油、胆固醇、肿瘤坏死因子-α(TNF-α)、脂联素,取脂肪匀浆测定TNF-α、脂联素。结果模型组体质量、IRI、胆固醇升高(P＜0.01),脂肪匀浆中脂联素降低(P＜0.01),血清和脂肪匀浆TNF-α升高(P＜0.01)；与模型组比较,DIO-R组治疗前后体质量、IRI、胆固醇均明显降低(P＜0.01),脂肪匀浆脂联素升高(P＜0.01)；西布曲明组治疗后体质量、胆固醇均明显降低(P＜0.01),血清和脂肪中TNF-α减低(P＜0.05)；升清组治疗后体质量、IRI、胆固醇降低(P＜0.05,P＜0.01),血清和脂肪匀浆中 TNF-α均减低(P＜0.05,P＜0.01),脂肪匀浆中脂联素升高(P＜0.05)；运脾组IRI、胆固醇和血清中TNF-α降低(P＜0.05,P＜0.01),血清和脂肪中脂联素升高(P＜0.05)；黄芪组体质指数、血糖、IRI、胆固醇降低(P＜0.05,P＜0.01),血清和脂肪中TNF-α降低、脂联素升高(P＜0.05,P＜0.01)。结论黄芪可以抑制高脂饲料诱导的肥胖,改善糖脂代谢紊乱和IR优于运脾、升清中药,其机制与降低TNF-α和升高脂联素水平相关。
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