目的 观察一指禅推拿对坐骨神经损伤大鼠神经形态及功能的影响.方法 暴露坐骨神经,用显微持针器钳夹坐骨神经制作坐骨神经损伤模型.SD大鼠随机分为假手术组、模型组和推拿组,假手术组暴露神经但不夹持.造模7 d后,推拿组大鼠一指禅推法治疗30 d,每日1次.30 d后,检测大鼠坐骨神经功能指数(SFI)、右下肢运动功能指数(BBB),HE染色、免疫组化检测和电镜观察大鼠患侧坐骨神经变化.结果 与模型组比较,推拿组可有效加快SFI恢复,升高BBB,促进坐骨神经形态结构恢复,S-100β 蛋白含量升高,促进新生神经超微结构生长及恢复.结论 一指禅推拿可有效促进大鼠坐骨神经损伤后神经形态及功能恢复.%Objective To study the effects of one finger massage on sciatic nerve injury rats. Methods The sciatic nerves were exposed, and the sciatic nerve was held by micro needles to make the sciatic nerve injury model. SD rats were randomly divided into sham-operation group, model group and massage group. In the sham-operation group, the sciatic nerves were exposed but not held. 7 d after the establishment of modeling, rats in massage group received one finger massage for 30 d. After 30 d, SFI and BBB of sciatic nerves were detected. HE staining, transmission electron microscope and immunochemistry assay were used to measure the changes of sciatic nerves. Results Compared with model group, massage group could speed up the recovery of SFI and increase BBB, promote the recovery of sciatic nerve morphology, increased protein level of S-100β, and enhance ultrastructure of newborn nerve growth and recovery. Conclusion One finger massage can effectively promote neurological and functional recovery after sciatic nerve injury in rats.
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