外源性LIF和IL-1对围着床期小鼠子宫内膜整合素β3亚基表达的调节

摘要

目的：研究外源性LIF及IL-1对围着床期子宫内膜整合素β 3表达的调节，探讨LIF参与着床的具体机制。方法：给孕2～4 d的小鼠注射不同浓度的白血病抑制因子、白介素- 1或白介素-1受体拮抗剂，应用免疫印迹法和RT-PCR法测定子宫内膜整合素β3的蛋白及 mRNA表达水平。结果：注射LIF、IL-1子宫内膜整合素β3的表达水平升高(P＜0 . 05)，且高剂量时升高更明显(P＜0.01)。注射IL-1ra整合素β3水平下降(P＜0 .05)。结论：细胞因子IL-1及LIF对围着床期子宫内膜表达整合素β3有促 调节作用，二者参与着床与调节细胞粘附分子表达有关。%Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin- 1(IL-1) on the expression of integrin β3 in the mouse endometrium during per i-implantation period and explore the mechanism which LIF participate in embryo ni c implantation.Methods:The mice were injected peritoneally with LIF,IL-1 or IL-1ra at different administration on day 2～4 of pregnancy .The level of integrin β3 protein was measured with Westernblot method and mR NA measured with RT-PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL-1(P＜0.05) and which reli ed o n administration.The level of integrin β3 mRNA was decreased by IL-1ra (P <0.05).Conclusion:LIF and IL-1 of cytokines can improve the expr ession of integrin β3 in the peri-implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.