HIV感染者调节性T细胞表面B、T淋巴细胞衰减因子的表达研究

摘要

Objective:To detect the expression of BTLA on Treg cells of HIV-infected patients and investigate the role of BTLA in HIV infection.Methods: Forty-four HIV-1-infected patients (twenty-four early HIV infection,fourteen chronic HIV-infected patients with CD4+ T counts> 200 cells/μl,AIDS patients with CD4+T counts<200 cells/μl) and nine healthy people served as normal controls were selected to detect the expression of BTLA on Treg cells by flow cytometry.The correlations between BTLA expression on Treg cells and disease progression or immune activation were studied.Results: There was a higher percentage of BTLA on Treg cells in chronic HIV patients and AIDS patients than that in early HIV infected patients(P<0.05,P<0.01),and the expression of BTLA on Treg cells in AIDS patients was higher than that in normal controls(P<0.05).The expression of BTLA on Treg cells was negatively correlated with CD4+T lymphocyte counts and positively correlated with viral load (P<0.001,P<0.01).The percentage of BTLA on Treg cells was positively correlated with CD4+CD38+T lymphocytes and CD4+HLA-DR+T lymphocytes(P<0.001,P<0.001).Conclusion: Increased BTLA expression on HIV-infected Treg cells is associated with disease progression,suggesting that it may accelerate disease progression by enhancing Treg cells inhibitory function and may provide intervention information for HIV infection in the future.%目的:对HIV感染者调节性T细胞(Regulatory T cell,Treg)上B 、T淋巴细胞衰减因子(B and T lymphocyte attenuator,BTLA)的表达水平进行检测,并探讨其在HIV感染进程中的作用.方法:选取24例感染在一年之内的HIV早期感染者(Early HIV infected patients,EHI组)、14例感染超过一年的HIV慢性感染者(CD4+T计数>200 cells/μl,HIV组)、6例AIDS患者(CD4+T计数<200 cells/μl,AIDS组)和9例健康人作为对照,应用流式细胞仪检测不同时期感染者及健康对照者Treg细胞BTLA的表达水平,分析其与疾病进展及免疫活化的相关性.结果:随着HIV疾病进展,EHI组、HIV组及AIDS组Treg细胞BTLA表达水平依次升高,其中HIV组与AIDS组Treg细胞BTLA表达水平显著高于EHI组(P<0.05及P<0.01),AIDS组Treg细胞BTLA的表达水平高于健康对照(P<0.05);Treg细胞BTLA表达水平与CD4+T淋巴细胞计数呈负相关(P<0.001),与病毒载量呈正相关(P<0.01);Treg细胞BTLA表达水平与活化CD4+CD38+T淋巴细胞及CD4+HLA-DR+T淋巴细胞呈正相关(P<0.001,P<0.001).结论:HIV感染者Treg细胞BTLA表达升高,与疾病进展显著相关,提示其可能通过加强Treg细胞的抑制功能加速疾病进展,并为未来HIV感染的干预提供信息.