HPV L1壳蛋白的表达与宫颈病变的严重程度关系研究

摘要

目的：采用免疫组织化学法检测 HPV L1壳蛋白的表达，并进一步分析研究其与宫颈病变的严重程度的关系。方法：选取我院妇科就诊患者的宫颈脱落细胞标本529例，所有患者进行薄层液基细胞学检查，同时行宫颈活检并采用免疫组织化学法检测 HPV L1壳蛋白的表达，并且分析其与不同程度宫颈病变的相关性。结果：在慢性宫颈炎、CINⅠ、CINⅡ、CINⅢ和宫颈鳞癌中，HPV L1壳蛋白的阳性表达率分别为26.16％、60.76％、30.98％、16.54％、0％，CINⅠ组与 CINⅡ组、CINⅢ组和宫颈鳞癌组的 HPV L1壳蛋白阳性表达率之间差异性均具有统计学意义（P 均＜0.05）。在 NILM、ASCUS、LSIL、HSIL、SCC 中，HPV L1壳蛋白的阳性表达率分别为20.15％、35.29％、63.63％、18.39％、0％，HSIL 组与 LSIL 组的 HPV L1壳蛋白的阳性表达率之间的比较，差异有显著统计学意义（P 均＜0.05）。结论：HPV L1壳蛋白的表达随宫颈病变程度的加重而降低，表明 HPV L1壳蛋白表达的检测对癌前病变的筛查和预防有着重要意义。%Objectives:To study the relationship between the expression of HPV L1 protein shell and the severity of cervical lesions using immunohistochemical method.Methods:529 patients in the gynecologic endocrine clinic of our hospital were chosen.Cervical exfoliated cells specimens and thin layer liquid based cytology examina-tion were performed on all patients.Meanwhile,immunocytochemistry method was used to detect HPV L1 shell pro-tein expression and analyze its correlation with different degrees of cervical lesions.Results:In chronic cervicitis, CINⅠ,CINⅡ,CINⅢ and cervical adenocarcinoma,the positive expression rate of HPV L1 shell protein were 26.16%, 60.76%,30.98%,16.54% and 0% respectively.Difference in HPV L1 protein positive expression rate between CINⅠgroup and CINⅡ,CINⅢ and cervical adenocarcinoma was statistically significant (P <0.05).In NILM,AS-CUS,LSIL,HSIL and SCC,the HPV L1 shell protein positive expression rate were 20.15%,35.29%,63.63%, 1 8.39% and 0%,with statistically significant difference between HSIL and LSIL group (P <0.05).Conclusions:The expression of HPV L1 protein shell reduces with the increase of cervical lesion degree,suggesting that HPV L1 protein expression detection is of important significance in the screening and prevention of precancerous lesions.