西格列汀对2型糖尿病大鼠Nrf2表达调控及胰岛β细胞功能的影响

摘要

Objective To study the effect of sitagliptin on pancreatic beta cell function and the expression of Nrf 2 in type 2 diabetic rats.Methods 80maleWistarratswerefedfor1week,as20ratswereselectedasnormalcontrolgroupaccordingtotherandomnumberta-ble, then construct the model of type 2 diabetes mellitus.The remaining 60 rats were randomly divided into model and treatment groups , 20 rats in each group .After the success of modeling , the model group was orally administered with 0.9%saline, and the treatment group was given sitagliptin dissolved with 0.9%saline.Results After 20 weeks of treatment , the blood glucose level of treatment group was signifi-cantly lower than that of model group (P<0.05), serum insulin level was significantly higher than that of model group (P<0.05).The lev-els of HBCI, FBCI in treatment group were significantly higher than those in model group (P<0.05).The levels of serum MDA, TNF-alpha and MDA in pancreatic of treatment group were significantly lower than those in model group (P<0.05).The levels of T-SOD in serum and pancreas in treatment group were significantly higher than those in model group .The expression level of Nrf 2 of treatment group was signifi-cantly higher than that of model group (P<0.05).Conclusions Sitagliptin could upregulate Nrf2 expression in type 2 diabetic rats, im-prove the function of islet beta cells , which has definite clinical effect on type 2 diabetes.%目的 研究西格列汀对2型糖尿病大鼠Nrf2表达调控及胰岛β细胞功能的影响.方法 雄性Wistar大鼠80只适应性饲养1 w后,采用随机数字表选取20只胰岛β细胞功能指数作为正常对照组,其余60只构建2型糖尿病模型,造模成功后根据随机数字表分为模型组和治疗组,每组20只.模型组灌胃0.9%生理盐水,治疗组灌胃0.9%生理盐水配制的西格列汀,给药剂量为100 mg·kg-1·d-1,灌胃周期为20 w.对各组大鼠胰岛β 细胞功能,Nrf2表达水平进行分析.结果 干预20 w后,治疗组血糖水平显著低于模型组(P<0.05),血清胰岛素水平显著高于模型组(P<0.05).治疗组(HBCI)、胰岛β细胞分泌功能指数(FBCI)显著均高于模型组(P<0.05).治疗组大鼠血清和胰腺丙二醛(MDA)、肿瘤坏死因子(TNF)-α显著低于模型组(P<0.05).治疗组大鼠血清和胰腺总超氧化物歧化酶(T-SOD)均显著高于模型组(P<0.05).治疗组大鼠Nrf2表达水平显著高于模型组(P<0.05).结论 西格列汀可显著上调2型糖尿病大鼠Nrf2表达水平,改善其胰岛 β细胞功能,对2型糖尿病具有确切的临床疗效.