Objective To explore the effects of stromal cell-derived factor-1 ( SDF-1 ) on the hepatic injury recovery,and compare simple bone marrow mononuclear cellular transplant with SDF-1. Methods Hepatic injury mice models were established by carbon tetrachloride (CCl4) and adnunistration of 2-acetylminofluorene (2-AAF), and the bone marrow mononuclear cells (BMMNC) were isolated from the mouse' s bone marrow. Group A: SDF-1 was iniected into the abdominal cavity of the models. Group B: the bone marrow mononuclear cells were injected into the trail vein of the mouse models. Group C: normal saline was injected into the trail vein of the models. The same quantity of mouse were executed from each group in the second, the third and the fourth weeks. Additionally, the hepatic function (AST, ALT,TBil) and the hepatic tissue pathological section were detected to observe the difference of the hepatic injury recovery in each group. Results There was no significant difference of ALT (19.0 ±2.0 vs 19.7 ±4.7, P＞0. 05; 19.0 ±5.0 vs 14.5 ±2.5, P ＞0,05; 40.0 ± 17.0 vs 15.0 ±3.0, P ＞0.05); AST(117.1 ± 18.0 vs 116.7 ±20.0, P ＞0.05; 97.5 ±5.0 vs 104.5 ±23.5, P ＞0.05; 177.3 ±61.0 vs 105.0 ±49.1, P ＞0.05); TBil (1.8 ±0.1 vs 1.9 ±0.3, P ＞0.05; 1.75 ± 0.55 vs 1.5 ± 0.4, P ＞ 0.05; 1.8 ± 0.6 vs 1.5 ± 0. 3, P ＞ 0. 05 ) between group A and group B in 2 nd,3 rd and 4 th weeks. Conclusion Our study confirms that not only SDF-1 but also BMMNC can promote the hepatic injury recovery, especially the bone marrow mononuclear cells.%目的 探索基质细胞衍生因子对急性肝损伤修复的影响,并与单纯骨髓单个核细胞移植治疗肝损伤疗效相比较.方法 建立小鼠CCl4-AAF肝损伤模型,从小鼠骨髓分离骨髓单个核细胞.实验A组经腹腔向模型小鼠体内注入基质细胞衍生因子,实验B组经尾静脉向模型小鼠体内注入单个核细胞,对照C组经尾静脉向模型小鼠体内注入生理盐水,在第2周、3周、4周,从各组取出相同的小鼠处死,通过测肝功能指标(AST、ALT、Tbil)和肝组织病理切片,比较各组小鼠肝损伤修复的差异.结果 分别在第2周、3周、4周测得的SDF-1组(A组)和单个核细胞组(B组)的数值比较(U/L):ALT(第2周:19.0±2.0 vs 19.7±4.7,P>0.05;第3周:19.0±5.0 vs 14.5±2.5,P>0.05;第4周:40.0±17.0 vs 15.0±3.0,P＞0.05);AST(第2周:117.1±18.0 vs 116.7±20.0,P>0.05;第3周:97.5±5.0 vs 104.5±23.5,P>0.05;第4周:177.3±61.0 vs 105.0±49.1,P>0.05);Tbil(第2周:1.8±0.1 vs 1.9±0.3,P>0.05;第3周:1.75±0.55 vs 1.5±0.4,P>0.05;第4周:1.8±0.6 vs 1.5±0.3,P>0.05).在病理方面第2周、4周时A组及B组肝细胞仍有肿胀,但组织结构较急性损伤时有明显改善.结论 基质细胞衍生因子和单个核细胞都能够促进肝损伤的修复,在病理方面骨髓单个核细胞对损伤修复效果更明显一些.
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