快速进入高海拔地区小肠动力紊乱大鼠Cajal间质细胞及P物质表达的变化

摘要

目的 探讨快速进入高海拔地区发生小肠动力紊乱过程中Cajal间质细胞(interstitial cell of Cajal,ICC)的作用机制.方法 50只SD大鼠以低海拔地区(海拔400 m)为对照组,并以进入高海拔地区(海拔4300 m)后的时间节点依次分为1 d、3 d、7 d和14 d组,每组10只.测定各组大鼠小肠电活动数据,应用电子显微镜观察小肠ICC的微观结构变化,应用双重免疫荧光组织化学染色的方法 观察同区域ICC的功能与P物质(substance P,SP)的表达变化.结果 快速进入高海拔地区后,大鼠小肠电波幅及波频受损明显,于高海拔区3 d组下行到最低点(P<0.05).其小肠ICC的微观结构也可观察到类似改变.使用双重免疫荧光组织化学染色可发现,ICC与SP的表达呈同步变化,同样在高海拔区3 d组表达下降到最低点(P<0.05).结论 快速进入高海拔地区所致小肠动力紊乱的发生,可能与小肠ICC及SP的表达变化有密切关系.%Objective To explore the mechanism of interstitial cells of Cajal ( ICC) in rats with small intestine mo-tility disorder by high altitude exposed. Methods Fifty SD male rats were divided into five groups, control group was located in low altitude ( Xi' an, 400 m) , on the basis of the time points of entering high altitude ( Ma' duo, 4300 m) , rats were divided into four groups of 1 day, 3 days, 7 days and 14 days. Each group contained 10 rats. Rats' small in-testine electrical activity was measured in different groups. Electron microscopy was applied to observe the physiological ultrastructure of ICC, and the immunofluorescence staining was applied to observe the physiological function of ICC and the expression of substance P in small intestine. Results The amplitude and frequency of small intestine electrical ac-tivity of rats were lowest in high altitude 3 days group ( P<0 . 05 ) . The gap junctions and organelles of ICC were re-duced. The expressions of ICC and substance P were the lowest in high altitude 3 days group (P<0. 05). Conclusion The changes of the expressions of ICC and SP in small intestine are closely related to small intestine dynamic disorders in high altitude exposed rats.