镜鲤肌肉品质性状的微卫星标记分析

摘要

A total of 233 microsatellite markers were used to analyze the genotypes in the reference panel contained 107 individuals obtained from mirror common carp(Cyprinus carpio L.) strain, and two muscle quality traits including fat content and protein content were tested using GLM (General Linear Model) single marker of the regression. The determination of the threshold values by 10000 Permutation test revealed that 17 markers had significant correlation (P < 0.05) with the two traits, in which HLJ030, HLJ2560, HLJ3633 and HLJ3554 had very significant correlation with muscle protein content(P<0.01). In addition, the genotypes of these cor-relative loci captured were determined by Daucan’s in SPSS 17.0 software. These functional markers and genotypes related to the traits may provide the efficient evidence for improving muscle quality trait in the common carp.%利用233个微卫星标记检测镜鲤（Cyprinus carpio L.）一个家系的107尾个体的基因型，采用GLM方法对肌肉脂肪含量和蛋白质含量4种经济性状进行单标记回归分析。 Permutation检验结果显示：有17个标记分别与肌肉脂肪和蛋白质含量具有显著相关性（P<0.05），其中，有4个标记（HLJ030、HLJ2560、HLJ3633，和HLJ3554）与肌肉蛋白质含量呈极显著相关（P<0.01），表明这些标记与性状间可能存在连锁关系。对同一标记不同基因型之间进行了多重比较，找到了与这两种性状相关的优势基因型，为鲤的肌肉品质性状选育提供了有效的依据。