目的 观察高脂饮食对C57BL/6小鼠出现外周神经病变的影响. 方法 将3周龄雌性C57BL/6小鼠随机分为正常对照(NC)组和高脂饮食(HF)组,隔周行腹腔内葡萄糖耐量实验,于HF组小鼠开始出现糖耐量异常时行热痛觉及神经传导速度测定,并检测血清中脂质水平、总超氧化物歧化酶(T-SOD)活性和丙二醛(MDA)含量. 结果 分组喂养14周后,HF组小鼠出现糖耐量异常,热反应时间缩短,运动和感觉神经传导速度均明显减慢(P均＜0.05).TC、HDL-C、LDL-C分别较NC组升高86.99％、38.0％、85.42％(P均＜0.01),血清中T-SOD活性及MDA含量均显著升高(P均＜0.05).结论 高脂饮食可诱导C57BL/6小鼠发生外周神经病变,血脂紊乱及氧化应激可能是危险因素.%Objective To investigate the effect of high-fat diet on peripheral neuropathy in C57BL/6 mice. Methods Female C57BL/6 mice aged 3 weeks were randomly assigned to the control and high-fat diet (HFD) groups. An intraperitoneal glucose tolerance test was performed every other week. Measurements of thermal pain threshold and nerve conduction velocity (NCV) were taken when the HFD-fed mice displayed glucose intolerance. Lipid profile, total superoxide dismutase (T-SOD) activity and malondial-chelyche (MDA) content in serum were examined. Results Following 14 weeks on a high-fat diet, mice displayed glucose intolerance and significantly decreased values of thermal pain threshold, motor nerve conduction velocity (MNCV), and sensory nerve conduction velocity (SNCV) (all P<0. 05). The HFD-fed mice showed an increase in serum total cholesterol(TC), high density lipoprotein cholesterol (HDL-C), and low density lipoprotein cholesterol (LDL-C) respectively by 86. 99%, 38. 0%, and 85. 42% (all P< 0. 01) and in T-SOD activity and MDA content (P<0. 01) compared with mice on a control diet Conclusion High-fat diet produces peripheral neuropathy in C57BL/6 mice. Dyslipidemia and oxidative stress may be the risk factors.
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