Objective To observe the changes of Smad ubiquitin regulatory factor 2 (Smurf2) and transcription co-repressor SnoN expression in renal tissues of type 2 diabetic rats and their biological roles. Methods SD rats were injected with STZ after taking high fat and high sucrose diet for 12 weeks, and were killed thereafter at the 8th, 12th, and 16th weeks respectively. SnoN, Smurf2, TGF-β1, and Smad2 in renal cortex were detected by immunohistochemistry and Western blotting. SnoN mRNA was checked with RT-PCR. Results The expression of Smurf2 in diabetic renal tissues was significantly increased (P <0. 01), meanwhile the expression of SnoN was significantly decreased (P<0. 05), and there was a negative correlation between these two proteins (r= -0. 71, P<0. 01). SnoN mRNA in diabetic renal tissues was unchanged among groups (P > 0. 05). Conclusion The results suggest that the downregulation of SnoN expression may be mediated by Smurf2 in type 2 diabetic nephropathy.%目的 研究Smad泛素化调节因子2(Smurf2)和核转录共抑制因子(SnoN)在T2DM大鼠肾组织中表达的动态变化及意义.方法 建立T2DM模型,于成模8、12和16周处死.免疫组化和Western blot测肾组织Smurf2、SnoN、Ttal和Smad2蛋白的表达;RT-PCR测肾皮质SnoN mRNA.结果 DM大鼠肾组织Smurf2蛋白表达增多(P<0.01),而SnoN蛋白的表达随病程进展逐渐减少(P<0.05),两者呈显著负相关(r=-0.71,P<0.01);SnoN mRNA在各组肾组织中的表达差异无统计学意义(P>0.05).结论 T2DM肾病发病过程中,Smurf2蛋白的表达增加可能参与了SnoN蛋白的降解过程.
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