1,25二羟基维生素D3对2型糖尿病大鼠肝脏中维生素D受体、脂联素受体2、过氧化物酶体增殖物激活受体αmRNA表达及甘油三酯含量的影响

摘要

目的 探讨1,25二羟基维生素D3[1,25(OH)2D3]对T2DM大鼠肝脏的保护作用及机制.方法 100只雄性Wistar大鼠随机分成单纯T2DM组、1,25(OH)2D3干预组[1,25(OH)2D3 +DM]、过氧化物酶体增殖物激活受体α(PPAR-α)抑制组[1,25(OH)2D3 +PPAR-α抑制剂+DM]和正常对照(NC)组,每组各25只.6周后,测定各组FPG及血脂.ELISA检测肝脏组织中TG含量,RT-PCR检测大鼠肝脏组织中维生素D (VitD)受体(VDR)、PPAR-α、脂联素受体2(AdipoR2) mRNA表达水平.结果 1,25(OH)2D3 +DM组肝脏中VDR、PPAR-α、AdipoR2 mRNA表达较T2DM组增加(t=20.32,5.92,9.10,P＜0.05),TG含量较T2DM组减少(t=13.84,P＜0.05).结论 1,25(OH)2 D3可能通过上调VDR基因的表达,进而上调AdipoR2、PPAR-α基因表达,从而减少肝脏TG的含量.%Objective To explore the protective effect and mechanism of 1,25 dihydroxyvitamin D3 [1,25 (OH)2D3] on the liver of rats with type 2 diabetes (T2DM).Methods 100 male Wistar rats were randomly divided into four groups:T2DM group,Vitamin D(VitD) intervention group (VitD + DM group),peroxisome proliferator-activated receptors-alpha(PPAR-α) inhibitor group(VitD+DM+PPAR-α inhibitor group) and normal control group (NC group),with 25 in each group.After 6 weeks later,fasting serum glucose (FPG)and blood lipid were measured.The content of triglyceride(TG) in liver tissue was detected by enzyme method.The expressions of Vitamin D receptor (VDR),PPAR-α and adiponectin receptor 2 (AdipoR2) mRNA in rat liver was detected by real-time fluorescence quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results The expressions of VDR,PPAR-α and Adipo R2 mRNA were significantly increased in VitD intervention group compared with T2DM group (t=20.32,5.92,9.10;P＜0.05).The content of TG in VitD+T2DM group was significantly lower than that in T2DM group (t=13.84,P＜0.05).Conclusion 1,25 (OH)2 D3 may up-regulate the expression of VDR gene and then up-regulate the expressions of AdipoR2 and PPAR-α that decrease the content of TG in the liver.